ArticleHaematologica2014
GATA2 regulates differentiation of bone marrow-derived mesenchymal stem cells.
Article in Haematologica, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- Dissecting the impact of transcription factor dose on cell reprogramming heterogeneity using scTF-seq.Nature genetics · 2025Article
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- Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development.Cell reports · 2023Article
- Aplastic Anemia as a Roadmap for Bone Marrow Failure: An Overview and a Clinical Workflow.International journal of molecular sciences · 2022Review
- Single-cell Transcriptomic Analysis Reveals the Cellular Heterogeneity of Mesenchymal Stem Cells.Genomics, proteomics & bioinformatics · 2022Article
- T-Cell Death Associated Gene 51 Is a Novel Negative Regulator of PPARγ That Inhibits PPARγ-RXRα Heterodimer Formation in AdipogenesisMolecules and cells · 2021Article
- Deconstructing transcriptional variations and their effects on immunomodulatory function among human mesenchymal stromal cells.Stem cell research & therapy · 2021Article
- A Computational Model of the Endothelial to Mesenchymal Transition.Frontiers in genetics · 2020Article
- Obesity-Induced Changes in Bone Marrow Homeostasis.Frontiers in endocrinology · 2020Review
- Insensitive to PTH of CD8International journal of medical sciences · 2020Article
- Loss of the Hematopoietic Stem Cell Factor GATA2 in the Osteogenic Lineage Impairs Trabecularization and Mechanical Strength of Bone.Molecular and cellular biology · 2018Article
- Bone Marrow Fat and Hematopoiesis.Frontiers in endocrinology · 2018Review
- Automatic identification of informative regions with epigenomic changes associated to hematopoiesis.Nucleic acids research · 2017Article
- [The mechanism of bone marrow-derived mesenchymal stem cells excessive senescence in severe aplastic anemia mouse model].Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi · 2017Article
- GATA2 regulates dendritic cell differentiation.Blood · 2016Article
- Gata2 Is a Rheostat for Mesenchymal Stem Cell Fate in Male Mice.Endocrinology · 2016Article
- Subsets of Visceral Adipose Tissue Nuclei with Distinct Levels of 5-Hydroxymethylcytosine.PloS one · 2016Article
- The complex pathophysiology of acquired aplastic anaemia.Clinical and experimental immunology · 2015Review
- High-Throughput siRNA Screening to Reveal GATA-2 Upstream Transcriptional Mechanisms in Hematopoietic Cells.PloS one · 2015Article
- Article
Corrections and comments
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Authors and funding
13 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The bone marrow microenvironment comprises multiple cell niches derived from bone marrow mesenchymal stem cells. However, the molecular mechanism of bone marrow mesenchymal stem cell differentiation is poorly understood. The transcription factor GATA2 is indispensable for hematopoietic stem cell function as well as other hematopoietic lineages, suggesting that it may maintain bone marrow mesenchymal stem cells in an immature state and also contribute to their differentiation. To explore this possibility, we established bone marrow mesenchymal stem cells from GATA2 conditional knockout mice. Differentiation of GATA2-deficient bone marrow mesenchymal stem cells into adipocytes induced accelerated oil-drop formation. Further, GATA2 loss- and gain-of-function analyses based on human bone marrow mesenchymal stem cells confirmed that decreased and increased GATA2 expression accelerated and suppressed bone marrow mesenchymal stem cell differentiation to adipocytes, respectively. Microarray analysis of GATA2 knockdowned human bone marrow mesenchymal stem cells revealed that 90 and 189 genes were upregulated or downregulated by a factor of 2, respectively. Moreover, gene ontology analysis revealed significant enrichment of genes involved in cell cycle regulation, and the number of G1/G0 cells increased after GATA2 knockdown. Concomitantly, cell proliferation was decreased by GATA2 knockdown. When GATA2 knockdowned bone marrow mesenchymal stem cells as well as adipocytes were cocultured with CD34-positive cells, hematopoietic stem cell frequency and colony formation decreased. We confirmed the existence of pathological signals that decrease and increase hematopoietic cell and adipocyte numbers, respectively, characteristic of aplastic anemia, and that suppress GATA2 expression in hematopoietic stem cells and bone marrow mesenchymal stem cells.
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