Evidence map›Paper›PMID 25150061›Full record

ArticleAmerican journal of physiology. Lung cellular and molecular physiology2014

The genome-wide transcriptional response to neonatal hyperoxia identifies Ahr as a key regulator.

Soumyaroop Bhattacharya, Zhongyang Zhou, Min Yee, Chin-Yi Chu, Ashley M Lopez, Valerie A Lunger, Siva Kumar Solleti, Emily Resseguie, Bradley Buczynski, Thomas J Mariani and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in American journal of physiology. Lung cellular and molecular physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 42 citations in OpenAlex.

  1. Trial
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  8. Review
  9. Role of NRF2 in immune modulator expression in developing lung.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021
    Article
  10. Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Review
  17. Consequences of early postnatal lipopolysaccharide exposure on developing lungs in mice.American journal of physiology. Lung cellular and molecular physiology · 2019
    Article
  18. Lung omics signatures in a bronchopulmonary dysplasia and pulmonary hypertension-like murine model.American journal of physiology. Lung cellular and molecular physiology · 2018
    Article
  19. Review
  20. Mice Lacking the Cytochrome P450 1B1 Gene Are Less Susceptible to Hyperoxic Lung Injury Than Wild Type.Toxicological sciences : an official journal of the Society of Toxicology · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Soumyaroop BhattacharyaDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and.
Zhongyang ZhouDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York;
Min YeeDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Perinatal and Pediatric Origins of Disease Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York.
Chin-Yi ChuDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and.
Ashley M LopezDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and.
Valerie A LungerDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and.
Siva Kumar SolletiDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and.
Emily ResseguieDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Perinatal and Pediatric Origins of Disease Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York.
Bradley BuczynskiDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Perinatal and Pediatric Origins of Disease Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York.
Thomas J MarianiDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Pediatric Molecular and Personalized Medicine Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; and Tom_Mariani@URMC.rochester.edu.
Michael A O'ReillyDivision of Neonatology, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York; Perinatal and Pediatric Origins of Disease Program, Department of Pediatrics, University of Rochester Medical Center, Rochester, New York.
University of Rochester Medical Center · US

Funding

VISUAL INDICES OF NEUROTOXICITYP30ES001247 · NIEHS · UNIVERSITY OF ROCHESTER · PI Sally W Thurston · 1985 to 2026
$42.8M
Training in Environment ToxicologyT32ES007026 · NIEHS · UNIVERSITY OF ROCHESTER · PI Alison Elder, Marissa Sobolewski Terry · 1985 to 2026
$20.2M
Effect of Neonatal Hyperoxia on Alveolar Development and InfectionR01HL091968 · NHLBI · UNIVERSITY OF ROCHESTER · PI O'REILLY, MICHAEL A · 2009 to 2021
$5.4M
NHLBI NIH HHS R01 HL091968NIEHS NIH HHS P30 ES001247NIEHS NIH HHS T32 ES007026
6 · The paper itself

Abstract

Premature infants requiring supplemental oxygen are at increased risk for developing bronchopulmonary dysplasia (BPD). Rodent models involving neonatal exposure to excessive oxygen concentrations (hyperoxia) have helped to identify mechanisms of BPD-associated pathology. Genome-wide assessments of the effects of hyperoxia in neonatal mouse lungs could identify novel BPD-related genes and pathways. Newborn C57BL/6 mice were exposed to 100% oxygen for 10 days, and whole lung tissue RNA was used for high-throughput, sequencing-based transcriptomic analysis (RNA-Seq). Significance Analysis of Microarrays and Ingenuity Pathway Analysis were used to identify genes and pathways affected. Expression patterns for selected genes were validated by qPCR. Mechanistic relationships between genes were further tested in cultured mouse lung epithelial cells. We identified 300 genes significantly and substantially affected following acute neonatal hyperoxia. Canonical pathways dysregulated in hyperoxia lungs included nuclear factor (erythryoid-derived-2)-like 2-mediated oxidative stress signaling, p53 signaling, eNOS signaling, and aryl hydrocarbon receptor (Ahr) pathways. Cluster analysis identified Ccnd1, Cdkn1a, and Ahr as critical regulatory nodes in the response to hyperoxia, with Ahr serving as the major effector node. A mechanistic role for Ahr was assessed in lung epithelial cells, and we confirmed its ability to regulate the expression of multiple hyperoxia markers, including Cdkn1a, Pdgfrb, and A2m. We conclude that a global assessment of gene regulation in the acute neonatal hyperoxia model of BPD-like pathology has identified Ahr as one driver of gene dysregulation.

Indexed as

TranscriptomeAnimalsAnimals, NewbornBasic Helix-Loop-Helix ProteinsBronchopulmonary DysplasiaCell LineCluster AnalysisGene Expression RegulationGene Regulatory NetworksGenomeHumansHyperoxiaLungMiceMice, Inbred C57BLReceptors, Aryl HydrocarbonAhr protein, mouseBasic Helix-Loop-Helix ProteinsReceptors, Aryl Hydrocarbonaryl hydrocarbon receptorbronchopulmonary dysplasiahigh-throughput sequencing-based transcriptional profiling

Identifiers

PMID25150061
PMCPMC4280157
OpenAlexW2066861345

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.