ArticleApplied and environmental microbiology2014
Regulation of gene expression in diverse cyanobacterial species by using theophylline-responsive riboswitches.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 129 citations in OpenAlex.
- A set of constitutive promoters with graded strengths for gene expression in diverse cyanobacterial strains.Applied and environmental microbiology · 2026Article
- CRISPR-enhanced microalgae biosynthesis: a promising approach for future functional feed ingredients.Journal of animal science and biotechnology · 2026Review
- Harnessing engineered cyanobacteria for next-generation therapeutics.Archives of microbiology · 2026Review
- Circadian clock proteins KaiB and Rbp2 ofMicrobiology spectrum · 2026Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Toward predictable universal genetic circuit design.Quantitative biology (Beijing, China) · 2024Article
- Development ofFrontiers in microbiology · 2024Article
- A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteria.Nature communications · 2023Article
- Phenotypically complex living materials containing engineered cyanobacteria.Nature communications · 2023Article
- A portable regulatory RNA array design enables tunable and complex regulation across diverse bacteria.bioRxiv : the preprint server for biology · 2023Article
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing inACS synthetic biology · 2022Article
- Cyanobacterial secondary metabolites towards improved commercial significance through multiomics approaches.World journal of microbiology & biotechnology · 2022Review
- Generation of miniploid cells and improved natural transformation procedure for a model cyanobacteriumFrontiers in microbiology · 2022Article
- Sustainable citric acid production from COFrontiers in microbiology · 2022Article
- pSHDY: A New Tool for Genetic Engineering of Cyanobacteria.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Attenuator LRR - a regulatory tool for modulating gene expression in Gram-positive bacteria.Microbial biotechnology · 2021Article
- Approaches in the photosynthetic production of sustainable fuels by cyanobacteria using tools of synthetic biology.World journal of microbiology & biotechnology · 2021Review
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyanobacteria are photosynthetic bacteria that are currently being developed as biological production platforms. They derive energy from light and carbon from atmospheric carbon dioxide, and some species can fix atmospheric nitrogen. One advantage of developing cyanobacteria for renewable production of biofuels and other biological products is that they are amenable to genetic manipulation, facilitating bioengineering and synthetic biology. To expand the currently available genetic toolkit, we have demonstrated the utility of synthetic theophylline-responsive riboswitches for effective regulation of gene expression in four diverse species of cyanobacteria, including two recent isolates. We evaluated a set of six riboswitches driving the expression of a yellow fluorescent protein reporter in Synechococcus elongatus PCC 7942, Leptolyngbya sp. strain BL0902, Anabaena sp. strain PCC 7120, and Synechocystis sp. strain WHSyn. We demonstrated that riboswitches can offer regulation of gene expression superior to that of the commonly used isopropyl-β-d-thiogalactopyranoside induction of a lacI(q)-Ptrc promoter system. We also showed that expression of the toxic protein SacB can be effectively regulated, demonstrating utility for riboswitch regulation of proteins that are detrimental to biomass accumulation. Taken together, the results of this work demonstrate the utility and ease of use of riboswitches in the context of genetic engineering and synthetic biology in diverse cyanobacteria, which will facilitate the development of algal biotechnology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.