ArticleBMC microbiology2014
Microarray analysis of the transcriptional responses of Porphyromonas gingivalis to polyphosphate.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Contribution of -Omics Technologies in the Study ofInternational journal of molecular sciences · 2022Review
- Transcriptomic Profile Analysis ofEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Porphyromonas gingivalis adopts intricate and unique molecular mechanisms to survive and persist within the host: a critical update.Journal of oral microbiology · 2020Review
- Antibacterial effects of sodium tripolyphosphate againstJournal of veterinary science · 2019Article
- Gene expression of Porphyromonas gingivalis ATCC 33277 when growing in an in vitro multispecies biofilm.PloS one · 2019Article
- Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states.PloS one · 2017Article
- Gene expression changes in Porphyromonas gingivalis W83 after inoculation in rat oral cavity.BMC microbiology · 2015Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPolyphosphate (polyP) has bactericidal activity against a gram-negative periodontopathogen Porphyromonas gingivalis, a black-pigmented gram-negative anaerobic rod. However, current knowledge about the mode of action of polyP against P. gingivalis is incomplete. To elucidate the mechanisms of antibacterial action of polyP against P. gingivalis, we performed the full-genome gene expression microarrays, and gene ontology (GO) and protein-protein interaction network analysis of differentially expressed genes (DEGs).
resultsWe successfully identified 349 up-regulated genes and 357 down-regulated genes (>1.5-fold, P < 0.05) in P. gingivalis W83 treated with polyP75 (sodium polyphosphate, Na(n+2)P(n)O3(n+1); n = 75). Real-time PCR confirmed the up- and down-regulation of some selected genes. GO analysis of the DEGs identified distinct biological themes. Using 202 DEGs belonging to the biological themes, we generated the protein-protein interaction network based on a database of known and predicted protein interactions. The network analysis identified biological meaningful clusters related to hemin acquisition, energy metabolism, cell envelope and cell division, ribosomal proteins, and transposon function.
conclusionspolyP probably exerts its antibacterial effect through inhibition of hemin acquisition by the bacterium, resulting in severe perturbation of energy metabolism, cell envelope biosynthesis and cell division, and elevated transposition. Further studies will be needed to elucidate the exact mechanism by which polyP induces up-regulation of the genes related to ribosomal proteins. Our results will shed new light on the study of the antibacterial mechanism of polyP against other related bacteria belonging to the black-pigmented Bacteroides species.
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