ArticlePloS one2014
Cationic synthetic peptides: assessment of their antimicrobial potency in liquid preserved boar semen.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Resistance-guided isolation and identification of antibiotic-resistant bacteria from bull semen: exploring alternatives to antibiotics in semen extenders.Tropical animal health and production · 2026Article
- Perspectives in porcine semen conservation (liquid and frozen).The Journal of reproduction and development · 2026Review
- Effect of Antimicrobial Peptide BiF2_5K7K on Contaminated Bacteria Isolated from Boar Semen and Semen Qualities during Preservation and Subsequent Fertility Test on Pig Farm.Antibiotics (Basel, Switzerland) · 2024Article
- Label-free measurement of antimicrobial peptide interactions with lipid vesicles and nanodiscs using microscale thermophoresis.Scientific reports · 2023Article
- Growth Dynamic and Threshold Values for Spermicidal Effects of Multidrug-Resistant Bacteria in Extended Boar Semen.Microorganisms · 2023Article
- Bacteria and Boar Semen Storage: Progress and Challenges.Antibiotics (Basel, Switzerland) · 2022Review
- Candida Genus Maximum Incidence in Boar Semen Even after Preservation, Is It Not a Risk for AI though?Molecules (Basel, Switzerland) · 2022Article
- Antimicrobial Peptides Controlling Resistant Bacteria in Animal Production.Frontiers in microbiology · 2022Review
- Latest developments on the mechanism of action of membrane disrupting peptides.Biophysics reports · 2021Article
- Toxic and Microbiological Effects of Iron Oxide and Silver Nanoparticles as Additives on Extended Ram Semen.Animals : an open access journal from MDPI · 2021Article
- Iron Oxide Nanoparticles as an Alternative to Antibiotics Additive on Extended Boar Semen.Nanomaterials (Basel, Switzerland) · 2020Article
- Low temperature preservation of porcine semen: influence of short antimicrobial lipopeptides on sperm quality and bacterial load.Scientific reports · 2020Article
- Detection and characterization of Lactobacillus spp. in the porcine seminal plasma and their influence on boar semen quality.PloS one · 2018Article
- Immunological Tolerance, Pregnancy, and Preeclampsia: The Roles of Semen Microbes and the Father.Frontiers in medicine · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Various semen extender formulas are in use to maintain sperm longevity and quality whilst acting against bacterial contamination in liquid sperm preservation. Aminoglycosides are commonly supplemented to aid in the control of bacteria. As bacterial resistance is increasing worldwide, antimicrobial peptides (AMPs) received lively interest as alternatives to overcome multi-drug resistant bacteria. We investigated, whether synthetic cationic AMPs might be a suitable alternative for conventional antibiotics in liquid boar sperm preservation. The antibacterial activity of two cyclic AMPs (c-WWW, c-WFW) and a helical magainin II amide analog (MK5E) was studied in vitro against two Gram-positive and eleven Gram-negative bacteria. Isolates included ATCC reference strains, multi-resistant E. coli and bacteria cultured from boar semen. Using broth microdilution, minimum inhibitory concentrations were determined for all AMPs. All AMPs revealed activity towards the majority of bacteria but not against Proteus spp. (all AMPs) and Staphylococcus aureus ATCC 29213 (MK5E). We could also demonstrate that c-WWW and c-WFW were effective against bacterial growth in liquid preserved boar semen in situ, especially when combined with a small amount of gentamicin. Our results suggest that albeit not offering a complete alternative to traditional antibiotics, the use of AMPs offers a promising solution to decrease the use of conventional antibiotics and thereby limit the selection of multi-resistant strains.
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