ArticleJundishapur journal of microbiology2014
Cloning, Expression, and Purification of Recombinant Lysostaphin From Staphylococcus simulans.
Article in Jundishapur journal of microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 33 citations in OpenAlex.
- Molecular and genetic analysis of methicillin-resistant Staphylococcus aureus (MRSA) in a tertiary care hospital in Saudi Arabia.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
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- Cloning and expression ofAIMS microbiology · 2021Article
- Enzybiotics LYSSTAPH-S and LYSDERM-S as Potential Therapeutic Agents for Chronic MRSA Wound Infections.Antibiotics (Basel, Switzerland) · 2020Article
- A Rapid Lysostaphin Production Approach and a Convenient Novel Lysostaphin Loaded Nano-emulgel; As a Sustainable Low-Cost Methicillin-ResistantBiomolecules · 2020Article
- Production of Lysostaphin by Nonproprietary Method Utilizing a Promoter from Toxin-Antitoxin System.Molecular biotechnology · 2019Article
- Heterologous expression and purification of BtCspB, a novel cold-shock protein-like bacteriocin from Bacillus thuringiensis BRC-ZYR2.World journal of microbiology & biotechnology · 2019Article
- Synergistic Anti-Staphylococcal Activity Of Niosomal Recombinant Lysostaphin-LL-37.International journal of nanomedicine · 2019Article
- Production of Recombinant Antimicrobial Polymeric Protein Beta Casein-E 50-52 and Its Antimicrobial Synergistic Effects Assessment with Thymol.Molecules (Basel, Switzerland) · 2017Article
- Evolution of the Immune Response against Recombinant Proteins (TcpA, TcpB, and FlaA) as a Candidate Subunit Cholera Vaccine.Journal of immunology research · 2017Article
- In Vitro and in Vivo Antistaphylococcal Activity Determination of the New Recombinant Lysostaphin Protein.Jundishapur journal of microbiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStaphylococcus aureus is one of the most common causes of nosocomial infections and its resistance to antibiotics is a global concern. Lysostaphin is an antimicrobial agent belonging to a major class of antimicrobial peptides and proteins known as the bacteriocins. It exhibits a high degree of anti-staphylococcal bacteriolytic activity.
objectivesIn this study, high level of recombinant mature lysostaphin in Escherichia coli was produced by using pET32a expression vector. MATERIALS AND
methodsThe S. simulans gene encoding lysostaphin was extracted, amplified by polymerase chain reaction (PCR), and sub-cloned in prokaryotic expression vector pET32a. E. coli BL21 (DE3) plysS were transformed with pET32a-lys and gene expression was induced by IPTG. The expressed protein was purified by affinity-chromatography using (Ni-NTA) resin.
resultsPCR and sequencing results confirmed the successful cloning of the target gene into the vector. The expression of protein was induced by IPTG and high concentration of the recombinant protein was obtained via the purification process by affinity-chromatography.
conclusionsOur data showed that the recombinant mature lysostaphin protein produced by pET32a vector in E. coli system was very efficient.
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