ArticleFrontiers in microbiology2014
Methane production from protozoan endosymbionts following stimulation of microbial metabolism within subsurface sediments.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
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- Methanogenic symbionts of anaerobic ciliates are host and habitat specific.The ISME journal · 2024Article
- Detrimental impact of theMicrobiology spectrum · 2023Article
- Methane production bymBio · 2023Article
- Different outer membranemLife · 2022Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
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- Correlation of Key Physiological Properties ofApplied and environmental microbiology · 2021Article
- Ruminal Protozoal Populations of Angus Steers Differing in Feed Efficiency.Animals : an open access journal from MDPI · 2021Article
- The host-associated archaeome.Nature reviews. Microbiology · 2020Review
- Food selectivity of anaerobic protists and direct evidence for methane production using carbon from prey bacteria by endosymbiotic methanogen.The ISME journal · 2020Article
- A Membrane-Bound Cytochrome EnablesmBio · 2019Article
- Effect of the macroalgae Asparagopsis taxiformis on methane production and rumen microbiome assemblage.Animal microbiome · 2019Article
- Potential for Methanosarcina to Contribute to Uranium Reduction during Acetate-Promoted Groundwater Bioremediation.Microbial ecology · 2018Article
- Metatranscriptomic Evidence for Direct Interspecies Electron Transfer between Geobacter and Methanothrix Species in Methanogenic Rice Paddy Soils.Applied and environmental microbiology · 2017Article
- Effects of Predation by Protists on Prokaryotic Community Function, Structure, and Diversity in Anaerobic Granular Sludge.Microbes and environments · 2016Article
- Intra- and inter-species interactions in microbial communities.Frontiers in microbiology · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previous studies have suggested that protozoa prey on Fe(III)- and sulfate-reducing bacteria that are enriched when acetate is added to uranium contaminated subsurface sediments to stimulate U(VI) reduction. In order to determine whether protozoa continue to impact subsurface biogeochemistry after these acetate amendments have stopped, 18S rRNA and ß-tubulin sequences from this phase of an in situ uranium bioremediation field experiment were analyzed. Sequences most similar to Metopus species predominated, with the majority of sequences most closely related to M. palaeformis, a cilitated protozoan known to harbor methanogenic symbionts. Quantification of mcrA mRNA transcripts in the groundwater suggested that methanogens closely related to Metopus endosymbionts were metabolically active at this time. There was a strong correlation between the number of mcrA transcripts from the putative endosymbiotic methanogen and Metopus ß-tubulin mRNA transcripts during the course of the field experiment, suggesting that the activity of the methanogens was dependent upon the activity of the Metopus species. Addition of the eukaryotic inhibitors cyclohexamide and colchicine to laboratory incubations of acetate-amended subsurface sediments significantly inhibited methane production and there was a direct correlation between methane concentration and Metopus ß-tubulin and putative symbiont mcrA gene copies. These results suggest that, following the stimulation of subsurface microbial growth with acetate, protozoa harboring methanogenic endosymbionts become important members of the microbial community, feeding on moribund biomass and producing methane.
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