ArticleRNA (New York, N.Y.)2014
Clostridium difficile Hfq can replace Escherichia coli Hfq for most of its function.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Control of iron acquisition by multiple small RNAs unravels a new role for transcriptional terminator loops in gene regulation.Nucleic acids research · 2024Article
- Insights into the complementation potential of the extreme acidophile's orthologue in replacing Escherichia coli hfq gene-particularly in bacterial resistance to environmental stress.World journal of microbiology & biotechnology · 2024Article
- Small proteins in Gram-positive bacteria.FEMS microbiology reviews · 2023Article
- Multiple in vivo roles for the C-terminal domain of the RNA chaperone Hfq.Nucleic acids research · 2022Article
- Identification of RNAs bound by Hfq reveals widespread RNA partners and a sporulation regulator in the human pathogenRNA biology · 2021Article
- An RNA-centric global view ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- C-terminal domain of the RNA chaperone Hfq drives sRNA competition and release of target RNA.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Arginine Patch Predicts the RNA Annealing Activity of Hfq from Gram-Negative and Gram-Positive Bacteria.Journal of molecular biology · 2016Article
- Hfq: the flexible RNA matchmaker.Current opinion in microbiology · 2016Review
- Hfqs in Bacillus anthracis: Role of protein sequence variation in the structure and function of proteins in the Hfq family.Protein science : a publication of the Protein Society · 2015Article
- Tracking the Elusive Function of Bacillus subtilis Hfq.PloS one · 2015Article
- Pleiotropic role of the RNA chaperone protein Hfq in the human pathogen Clostridium difficile.Journal of bacteriology · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A gene for the Hfq protein is present in the majority of sequenced bacterial genomes. Its characteristic hexameric ring-like core structure is formed by the highly conserved N-terminal regions. In contrast, the C-terminal forms an extension, which varies in length, lacks homology, and is predicted to be unstructured. In Gram-negative bacteria, Hfq facilitates the pairing of sRNAs with their mRNA target and thus affects gene expression, either positively or negatively, and modulates sRNA degradation. In Gram-positive bacteria, its role is still poorly characterized. Numerous sRNAs have been detected in many Gram-positive bacteria, but it is not yet known whether these sRNAs act in association with Hfq. Compared with all other Hfqs, the C. difficile Hfq exhibits an unusual C-terminal sequence with 75% asparagine and glutamine residues, while the N-terminal core part is more conserved. To gain insight into the functionality of the C. difficile Hfq (Cd-Hfq) protein in processes regulated by sRNAs, we have tested the ability of Cd-Hfq to fulfill the functions of the E. coli Hfq (Ec-Hfq) by examining various functions associated with Hfq in both positive and negative controls of gene expression. We found that Cd-Hfq substitutes for most but not all of the tested functions of the Ec-Hfq protein. We also investigated the role of the C-terminal part of the Hfq proteins. We found that the C-terminal part of both Ec-Hfq and Cd-Hfq is not essential but contributes to some functions of both the E. coli and C. difficile chaperons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.