ArticleMolecular human reproduction2015
Use of DEAD-box polypeptide-4 (Ddx4) gene promoter-driven fluorescent reporter mice to identify mitotically active germ cells in post-natal mouse ovaries.
Article in Molecular human reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 53 citations in OpenAlex.
- Role of Ovarian Stem Cells in Postnatal Oogenesis and Implications in Fertility Preservation.Advances in experimental medicine and biology · 2025Review
- Reconstruction of ovarian follicular-like structure by recellularization of a cell-free human ovarian scaffold with mouse fetal ovarian cells.Cytotechnology · 2024Article
- Mitochondrial Transfer into Human Oocytes Improved Embryo Quality and Clinical Outcomes in Recurrent Pregnancy Failure Cases.International journal of molecular sciences · 2023Article
- VASA protein and gene expression analysis of human non-obstructive azoospermia and normal by immunohistochemistry, immunocytochemistry, and bioinformatics analysis.Scientific reports · 2022Article
- Ovarian Stem Cells (OSCs) from the Cryopreserved Ovarian Cortex: A Potential for Neo-Oogenesis in Women with Cancer-Treatment Related Infertility: A Case Report and a Review of Literature.Current issues in molecular biology · 2022Review
- The process of ovarian aging: it is not just about oocytes and granulosa cells.Journal of assisted reproduction and genetics · 2022Review
- Offspring production of haploid spermatid-like cells derived from mouse female germline stem cells with chromatin condensation.Cell & bioscience · 2022Article
- Non-neutral clonal selection and its potential role in mammalian germline stem cell dysfunction with advancing age.Frontiers in cell and developmental biology · 2022Review
- Germ cells of the mammalian female: A limited or renewable resource?†.Biology of reproduction · 2021Review
- Spermidine induces cytoprotective autophagy of female germline stem cells in vitro and ameliorates aging caused by oxidative stress through upregulated sequestosome-1/p62 expression.Cell & bioscience · 2021Article
- Human Ovarian Cortex biobanking: A Fascinating Resource for Fertility Preservation in Cancer.International journal of molecular sciences · 2020Review
- Article
- GAS5/miR-21 Axis as a Potential Target to Rescue ZCL-082-Induced Autophagy of Female Germline Stem Cells In Vitro.Molecular therapy. Nucleic acids · 2019Article
- Article
- Extracellular matrix signaling activates differentiation of adult ovary-derived oogonial stem cells in a species-specific manner.Fertility and sterility · 2019Article
- Review
- Female Fertility Preservation through Stem Cell-based Ovarian Tissue Reconstitution In Vitro and Ovarian Regeneration In Vivo.Clinical medicine insights. Reproductive health · 2019Review
- Stem Cells, Self-Renewal, and Lineage Commitment in the Endocrine System.Frontiers in endocrinology · 2019Review
- Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019Article
- Cadherin 22 participates in the self-renewal of mouse female germ line stem cells via interaction with JAK2 and β-catenin.Cellular and molecular life sciences : CMLS · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Several laboratories have independently isolated mitotically active germ cells, termed female germline stem cells or oogonial stem cells (OSCs), from adult mouse ovaries. However, a recent study using Ddx4-Cre;Rosa26 reporter mice concluded that such germ cells do not exist. Given the disparity in conclusions drawn in this study compared with others, we felt it was important to re-assess the utility of Ddx4-Cre;Rosa26 reporter mice for identification of OSCs in adult mouse ovaries. Transgenic Ddx4-Cre mice were crossed with Rosa26(tdTm/tdTm) mice to drive restricted tomato red (tdTm) gene expression in cells in which the Ddx4 gene promoter has been activated. Crude dispersion of ovaries from recombined offspring generated cell fractions containing tdTm-positive immature oocytes, which are incapable of proliferation and thus probably represent the uncharacterized reporter-positive ovarian cells identified in the paper Zhang et al. (2012) as being mitotically inactive. Dispersed ovaries further subjected to fluorescence-activated cell sorting yielded a large population of non-germline tdTm-positive cells, indicative of promoter 'leakiness' in the Ddx4-Cre mouse line. Nonetheless, a small percentage of these tdTm-positive cells exhibited externalized (extracellular, ec) expression of Ddx4 protein (ecDdx4-positive), expressed markers of primitive germ cells but not of oocytes, and actively proliferated in culture, all of which are characteristic features of OSCs. Thus, crude dispersion of ovaries collected from Ddx4 gene promoter-driven reporter mice is not, by itself, a reliable approach to identify OSCs, whereas the same ovarian dispersates further subjected to cell sorting strategies yield purified OSCs that can be expanded in culture.
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