Evidence map›Paper›PMID 25146974›Full record

ArticleMolecular & cellular proteomics : MCP2014

Global proteome analysis identifies active immunoproteasome subunits in human platelets.

Cordula Klockenbusch, Geraldine M Walsh, Lyda M Brown, Michael D Hoffman, Vladimir Ignatchenko, Thomas Kislinger, Juergen Kast

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. Article
  3. An Insight into Recent Advances on Platelet Function in Health and Disease.International journal of molecular sciences · 2022
    Review
  4. Review
  5. Review
  6. Review
  7. Article
  8. Proteomics: A Tool to Study Platelet Function.International journal of molecular sciences · 2021
    Review
  9. The Role of the Proteasome in Platelet Function.International journal of molecular sciences · 2021
    Review
  10. Article
  11. Article
  12. Article
  13. Article
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Cordula KlockenbuschFrom the ‡The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada;
Geraldine M WalshFrom the ‡The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; §The Centre for Blood Research, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada;
Lyda M BrownFrom the ‡The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada;
Michael D HoffmanFrom the ‡The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; ¶The Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, BC V6T 1Z1, Canada;
Vladimir Ignatchenko‖Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C4, Canada;
Thomas Kislinger‖Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2C4, Canada; **Department of Medical Biophysics, University of Toronto, Toronto, ON M5G 1L7, Canada.
Juergen KastFrom the ‡The Biomedical Research Centre, 2222 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; §The Centre for Blood Research, 2350 Health Sciences Mall, University of British Columbia, Vancouver, BC V6T 1Z3, Canada; ¶The Department of Chemistry, 2036 Main Mall, University of British Columbia, Vancouver, BC V6T 1Z1, Canada; Kast@brc.ubc.ca.

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

The discovery of new functions for platelets, particularly in inflammation and immunity, has expanded the role of these anucleate cell fragments beyond their primary hemostatic function. Here, four in-depth human platelet proteomic data sets were generated to explore potential new functions for platelets based on their protein content and this led to the identification of 2559 high confidence proteins. During a more detailed analysis, consistently high expression of the proteasome was discovered, and the composition and function of this complex, whose role in platelets has not been thoroughly investigated, was examined. Data set mining resulted in identification of nearly all members of the 26S proteasome in one or more data sets, except the β5 subunit. However, β5i, a component of the immunoproteasome, was identified. Biochemical analyses confirmed the presence of all catalytically active subunits of the standard 20S proteasome and immunoproteasome in human platelets, including β5, which was predominantly found in its precursor form. It was demonstrated that these components were assembled into the proteasome complex and that standard proteasome as well as immunoproteasome subunits were constitutively active in platelets. These findings suggest potential new roles for platelets in the immune system. For example, the immunoproteasome may be involved in major histocompatibility complex I (MHC I) peptide generation, as the MHC I machinery was also identified in our data sets.

Indexed as

Blood PlateletsCell Line, TumorData MiningGene ExpressionHEK293 CellsHistocompatibility Antigens Class IHumansImmunity, InnateMolecular Sequence AnnotationPrimary Cell CultureProteasome Endopeptidase ComplexProtein SubunitsProteomeATP dependent 26S proteaseHistocompatibility Antigens Class IProteasome Endopeptidase ComplexProtein SubunitsProteome

Identifiers

PMID25146974
PMCPMC4256485

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.