ArticleJournal of visualized experiments : JoVE2014
High-throughput assay to phenotype Salmonella enterica Typhimurium association, invasion, and replication in macrophages.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- RANKL inhibits macrophage proinflammatory Toll-like receptor 2 and 4 signaling and impairs killing of intracellular bacteria.ImmunoHorizons · 2026Article
- A low-endotoxic Salmonella vector with dual bacterial-host promoter expression of Lawsonia intracellularis antigens elicits protective immunity in a murine model.Veterinary research · 2026Article
- Development and characterization of immortalized mouse intestinal epithelial cell lines.Scientific reports · 2026Article
- Genomic characterization of a virulent multidrug-resistant Salmonella enterica serovar Enteritidis strain isolated from meat rabbits (Oryctolagus cuniculus) in China.BMC microbiology · 2026Article
- Asiatic acid, a novel ciprofloxacin adjuvant inhibitsGut microbes · 2025Article
- Indispensable role of AcrEF in modulating Salmonella virulence and disrupting host tight junctions to facilitate paracellular entry and invasion.Biology direct · 2025Article
- Arginine-mediated inhibition of macrophage apoptosis byFrontiers in immunology · 2025Article
- Article
- Intracellular Survival and Pathogenicity Modulation ofInternational journal of molecular sciences · 2024Article
- Genomic and phenotypic characterization ofMicrobial genomics · 2023Article
- Polyimidazolium Protects against an Invasive Clinical Isolate of Salmonella Typhimurium.Antimicrobial agents and chemotherapy · 2022Article
- Effect of Hcp Iron Ion Regulation on the Interaction BetweenFrontiers in cellular and infection microbiology · 2022Article
- Identification of Natural Mutations Responsible for Altered Infection Phenotypes of Salmonella enterica Clinical Isolates by Using Cell Line Infection Screens.Applied and environmental microbiology · 2021Article
- TargetingFrontiers in microbiology · 2021Article
- The Differential Phosphorylation-Dependent Signaling and Glucose Immunometabolic Responses Induced during Infection byMicroorganisms · 2020Article
- Combining Whole-Genome Sequencing and Multimodel Phenotyping To Identify Genetic Predictors ofmSphere · 2020Article
- Influence of Heterologously Expressed azurin from Pseudomonas aeruginosa on the Adhesion and Invasion of Pathogenic Bacteria to the Caco-2 Cell Line.Probiotics and antimicrobial proteins · 2020Article
- Characterization of splenic MRC1Veterinary research · 2020Article
- Iron Limitation inFrontiers in microbiology · 2020Article
- Inhibition of the type III secretion system by syringaldehyde protects mice from Salmonella enterica serovar Typhimurium.Journal of cellular and molecular medicine · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Salmonella species are zoonotic pathogens and leading causes of food borne illnesses in humans and livestock. Understanding the mechanisms underlying Salmonella-host interactions are important to elucidate the molecular pathogenesis of Salmonella infection. The Gentamicin protection assay to phenotype Salmonella association, invasion and replication in phagocytic cells was adapted to allow high-throughput screening to define the roles of deletion mutants of Salmonella enterica serotype Typhimurium in host interactions using RAW 264.7 murine macrophages. Under this protocol, the variance in measurements is significantly reduced compared to the standard protocol, because wild-type and multiple mutant strains can be tested in the same culture dish and at the same time. The use of multichannel pipettes increases the throughput and enhances precision. Furthermore, concerns related to using less host cells per well in 96-well culture dish were addressed. Here, the protocol of the modified in vitro Salmonella invasion assay using phagocytic cells was successfully employed to phenotype 38 individual Salmonella deletion mutants for association, invasion and intracellular replication. The in vitro phenotypes are presented, some of which were subsequently confirmed to have in vivo phenotypes in an animal model. Thus, the modified, standardized assay to phenotype Salmonella association, invasion and replication in macrophages with high-throughput capacity could be utilized more broadly to study bacterial-host interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.