ArticlePlant cell reports2014
Xcc-facilitated agroinfiltration of citrus leaves: a tool for rapid functional analysis of transgenes in citrus leaves.
Article in Plant cell reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 79 citations in OpenAlex.
- The impact of CsPSKs on plant immunity: insights from a genome-wide study of small peptides in Citrus sinensis.Functional & integrative genomics · 2026Article
- Rye sprout as a host for the rapid transient production of a recombinant protein using a geminivirus DNA-containing expression vector.Plant biotechnology (Tokyo, Japan) · 2025Article
- CRISPR/Cas9-Targeted Gene Editing of Allergenic Profilin-EncodingPlants (Basel, Switzerland) · 2025Article
- CRISPR/Cas9 -based genome editing to expedite the genetic improvement of palms: challenges and prospects.Frontiers in plant science · 2024Article
- Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome.Frontiers in plant science · 2024Article
- Generation of transgene-free canker-resistantFrontiers in plant science · 2024Article
- Applications of CRISPR/Cas genome editing in economically important fruit crops: recent advances and future directions.Molecular horticulture · 2023Review
- Review
- LbCas12a-D156R Efficiently EditsCells · 2022Article
- Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits.Frontiers in plant science · 2022Review
- Base Editors for Citrus Gene Editing.Frontiers in genome editing · 2022Article
- Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content.Genes · 2021Review
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- A simple and efficient agroinfiltration method for transient gene expression in Citrus.Plant cell reports · 2021Article
- Induced Genetic Variations in Fruit Trees Using New Breeding Tools: Food Security and Climate Resilience.Plants (Basel, Switzerland) · 2021Review
- The transcriptome landscapes of citrus leaf in different developmental stages.Plant molecular biology · 2021Article
- Approach for in vivo delivery of CRISPR/Cas system: a recent update and future prospect.Cellular and molecular life sciences : CMLS · 2021Review
- Consequences of adaptation of TAL effectors on host susceptibility to Xanthomonas.PLoS genetics · 2021Article
- Citrus Genetic Transformation: An Overview of the Current Strategies and Insights on the New Emerging Technologies.Frontiers in plant science · 2021Review
- The Genetic Components of a Natural Color Palette: A Comprehensive List of Carotenoid Pathway Mutations in Plants.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageXanthomonas citri subsp. citri pretreatment before agroinfiltration could significantly promote transient expression in citrus leaves which were previously recalcitrant to agroinfiltration. Transient expression via agroinfiltration is widely used in biotechnology but remains problematic in many economically important plants. Xanthomonas citri subsp. citri (Xcc)-facilitated agroinfiltration was employed to promote transient protein expression in Valencia sweet orange leaves, which are recalcitrant to agroinfiltration. However, it is unclear whether Xcc-facilitated agroinfiltration has broad application, i.e., whether Xcc-facilitated agroinfiltration could be used on other citrus varieties. In addition, we intended to investigate whether Xcc-facilitated agroinfiltration could be used to hasten transgene function assays, e.g., Cre/lox system and Cas9/sgRNA system. In this report, Xcc-facilitated agroinfiltration was further exploited to enhance β-glucuronidase (GUS) expression in five citrus varieties. Xcc-facilitated agroinfiltration also significantly increased GFP expression in six citrus varieties tested. Both GUS and GFP assays indicated that Xcc-facilitated agroinfiltration had the best performance in grapefruit. After Xcc-facilitated agroinfiltration was carried out in grapefruit, protoplast analysis of the transformed cells indicated that there were more than 20 % leaf cells expressing GFP. In grapefruit, usefulness of Xcc-facilitated agroinfiltration was assayed in three case studies: (1) fast functional analysis of Cre/lox system, (2) the heat shock regulation of HSP70B promoter derived from Arabidopsis, and (3) Cas9/sgRNA-mediated genome modification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.