ArticleThe Journal of general virology2014
Characterization of a novel insect-specific flavivirus from Brazil: potential for inhibition of infection of arthropod cells with medically important flaviviruses.
Article in The Journal of general virology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
103 citing papers in PubMed, 1 synthesis or guideline pooled it, 159 citations in OpenAlex.
- Pooled it
- Transcriptomic and RNAi-mediated antiviral response to Kamiti River virus infection in Aedes albopictus U4.4 cells.Scientific reports · 2026Article
- Diversity of insect-specific viruses in Culex mosquitoes from Papua New Guinea.Virology journal · 2026Article
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- Differences in metavirome among Aedes albopictus, Culex tritaeniorhynchus, and Anopheles sinensis in Jiangxi Province, China.Parasites & vectors · 2026Article
- Unveiling the RNA virus landscape ofVirus evolution · 2026Article
- RNA metagenomic profiling of mosquito viromes associated with Vector-Borne diseases in Quebec, Canada.PloS one · 2026Article
- Diversity and persistence of the RNA virome of Philaenus spumarius, the European vector of Xylella fastidiosa.Scientific reports · 2025Article
- Systemic infection with insect-specific viruses does not affect Plasmodium sporozoite formation in Anopheles mosquitoes.PLoS neglected tropical diseases · 2025Article
- Characterization of two novel Negevirus strains and analysis of global Negevirus distribution and host diversity.Virology journal · 2025Article
- Microbiome diversity in mosquitoes and sand flies: implications for vector competence.Parasites & vectors · 2025Review
- Review
- Differential effect of acuteOne health (Amsterdam, Netherlands) · 2025Article
- Spatiotemporal prevalence and characterization of the lineage I insect-specific flavivirus, Quang Binh virus, isolated fromThe Journal of general virology · 2025Article
- An insect symbiotic virus promotes the transmission of a phytoarbovirus via inhibiting E3 ubiquitin ligase Sina.PLoS pathogens · 2025Article
- Characterisation of the virome ofThe Journal of general virology · 2025Article
- Insect-specific viruses: transmission dynamics and biological control strategies against arboviruses.Frontiers in microbiology · 2025Review
- Detection of Furrundu phlebovirus in Aedes scapularis (Diptera: Culicidae) collected in urban parks, in a highly urbanized city.Revista do Instituto de Medicina Tropical de Sao Paulo · 2025Article
- Review
43 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In the past decade, there has been an upsurge in the number of newly described insect-specific flaviviruses isolated pan-globally. We recently described the isolation of a novel flavivirus (tentatively designated 'Nhumirim virus'; NHUV) that represents an example of a unique subset of apparently insect-specific viruses that phylogenetically affiliate with dual-host mosquito-borne flaviviruses despite appearing to be limited to replication in mosquito cells. We characterized the in vitro growth potential and 3' untranslated region (UTR) sequence homology with alternative flaviviruses, and evaluated the virus's capacity to suppress replication of representative Culex spp.-vectored pathogenic flaviviruses in mosquito cells. Only mosquito cell lines were found to support NHUV replication, further reinforcing the insect-specific phenotype of this virus. Analysis of the sequence and predicted RNA secondary structures of the 3' UTR indicated NHUV to be most similar to viruses within the yellow fever serogroup and Japanese encephalitis serogroup, and viruses in the tick-borne flavivirus clade. NHUV was found to share the fewest conserved sequence elements when compared with traditional insect-specific flaviviruses. This suggests that, despite apparently being insect specific, this virus probably diverged from an ancestral mosquito-borne flavivirus. Co-infection experiments indicated that prior or concurrent infection of mosquito cells with NHUV resulted in a significant reduction in virus production of West Nile virus (WNV), St Louis encephalitis virus (SLEV) and Japanese encephalitis virus. The inhibitory effect was most effective against WNV and SLEV with over a 10(6)-fold and 10(4)-fold reduction in peak titres, respectively.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.