ArticleJournal of visualized experiments : JoVE2014
Preparation of rat serum suitable for mammalian whole embryo culture.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A Simple Mammalian Whole Embryo Culture System to Study High-Temperature Effects on Development.Acta histochemica et cytochemica · 2026Article
- Low-toxicity vitrification of small-diameter arterial grafts by achieving a Cryo-Stasis state.Materials today. Bio · 2026Article
- Ectomesenchyme contributes to epidermal stem cell formation through mesenchymal-to-epithelial transition.Nature communications · 2026Article
- Early coordination of cell migration and cardiac fate determination during mammalian gastrulation.The EMBO journal · 2025Article
- Using Ex Vivo Live Imaging to Investigate Cell Divisions and Movements During Mouse Dental Renewal.Journal of visualized experiments : JoVE · 2023Article
- Morphological phenotyping after mouse whole embryo culture.Frontiers in cell and developmental biology · 2023Review
- Live-Imaging Analysis of Epithelial Zippering During Mouse Neural Tube Closure.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Inhibitory effects ofFrontiers in immunology · 2023Article
- Bioreactors, scaffolds and microcarriers andFrontiers in nutrition · 2023Review
- Continuous live imaging reveals a subtle pathological alteration with cell behaviors in congenital heart malformation.Fundamental research · 2022Article
- miR-125a-5p inhibits glycolysis by targeting hexokinase-II to improve pulmonary arterial hypertension.Aging · 2020Article
- First steps in the development of a liquid biopsy in situ hybridization protocol to determine circular RNA biomarkers in rat biofluids.Pediatric surgery international · 2019Article
- Mouse whole embryo culture: Evaluating the requirement for rat serum as culture medium.Birth defects research · 2019Article
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Authors and funding
4 authors.
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Abstract
Mammalian whole embryo culture (WEC) is a widely used technique for examining pharmacological toxicity in developing mouse and rat embryos and for investigating the mechanisms of developmental processes. Immediately centrifuged (IC) rat serum is commonly used for WEC and is essential for the growth and development of cultured mouse and rat embryos ex vivo. For the culture of midgestation embryos (i.e., E8.0-12.5 for the mouse, and E10.0-14.5 for the rat), 100% rat serum is the best media for supporting the growth of the embryo ex vivo. To prepare rat serum suitable for WEC, the collected blood should be centrifuged immediately to separate the blood cells from the plasma fraction. After centrifugation, the fibrin clot forms in the upper layer; this clot should be squeezed gently using a pair of sterile forceps and subsequently centrifuged to completely separate the blood cells from the serum. In this video article, we demonstrate our standard protocol for the preparation of optimal IC rat serum, including blood collection from the abdominal aorta of male rats and extraction of the serum by centrifugation.
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