Evidence map›Paper›PMID 25145787›Full record

ArticleJournal of visualized experiments : JoVE2014

Promotion of survival and differentiation of neural stem cells with fibrin and growth factor cocktails after severe spinal cord injury.

Paul Lu, Lori Graham, Yaozhi Wang, Di Wu, Mark Tuszynski

Open access · bronzeAbstract readVideo-Audio Media
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
2.0field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 59 citations in OpenAlex.

  1. Article
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  7. Review
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  10. Review
  11. Cell transplantation to repair the injured spinal cord.International review of neurobiology · 2022
    Article
  12. Alginate hydrogel cross-linked by CaRegenerative biomaterials · 2022
    Article
  13. Article
  14. NFAT5 Deficiency Alleviates Formalin-Induced Inflammatory Pain Through mTOR.International journal of molecular sciences · 2021
    Article
  15. Article
  16. Review
  17. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Paul LuVeterans Administration Medical Center, San Diego; Department of Neurosciences, University of California, San Diego; plu@ucsd.edu.
Lori GrahamDepartment of Neurosciences, University of California, San Diego.
Yaozhi WangDepartment of Neurosciences, University of California, San Diego.
Di WuDepartment of Neurosciences, University of California, San Diego.
Mark TuszynskiVeterans Administration Medical Center, San Diego; Department of Neurosciences, University of California, San Diego.
University of California, San Diego · USUnited States Department of Veterans Affairs · US

Funding

MINORITY PREDOCTORAL FELLOWSHIP PROGRAM--NIGMSF31NS009881 · NINDS · UNIVERSITY OF TEXAS EL PASO · PI BOGGIANO, MARY M · 1994 to 1997
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NINDS NIH HHS F31 NS009881NINDS NIH HHS NS09881
6 · The paper itself

Abstract

Neural stem cells (NSCs) can self-renew and differentiate into neurons and glia. Transplanted NSCs can replace lost neurons and glia after spinal cord injury (SCI), and can form functional relays to re-connect spinal cord segments above and below a lesion. Previous studies grafting neural stem cells have been limited by incomplete graft survival within the spinal cord lesion cavity. Further, tracking of graft cell survival, differentiation, and process extension had not been optimized. Finally, in previous studies, cultured rat NSCs were typically reported to differentiate into glia when grafted to the injured spinal cord, rather than neurons, unless fate was driven to a specific cell type. To address these issues, we developed new methods to improve the survival, integration and differentiation of NSCs to sites of even severe SCI. NSCs were freshly isolated from embryonic day 14 spinal cord (E14) from a stable transgenic Fischer 344 rat line expressing green fluorescent protein (GFP) and were embedded into a fibrin matrix containing growth factors; this formulation aimed to retain grafted cells in the lesion cavity and support cell survival. NSCs in the fibrin/growth factor cocktail were implanted two weeks after thoracic level-3 (T3) complete spinal cord transections, thereby avoiding peak periods of inflammation. Resulting grafts completely filled the lesion cavity and differentiated into both neurons, which extended axons into the host spinal cord over remarkably long distances, and glia. Grafts of cultured human NSCs expressing GFP resulted in similar findings. Thus, methods are defined for improving neural stem cell grafting, survival and analysis of in vivo findings.

Indexed as

AnimalsCell DifferentiationCell SurvivalFemaleFibrinHumansIntercellular Signaling Peptides and ProteinsMaleNeural Stem CellsRatsRats, Inbred F344Rats, TransgenicSpinal Cord InjuriesStem Cell TransplantationFibrinIntercellular Signaling Peptides and Proteins

Identifiers

PMID25145787
PMCPMC4435462
OpenAlexW1983881747

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.