ArticleMicrobial cell factories2014
Metabolic engineering and classical selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation.
Article in Microbial cell factories, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 61 citations in OpenAlex.
- Positive selection of efficient ethanol producers from xylose at 45 °C in the yeast Ogataea polymorpha.Scientific reports · 2025Article
- Recent progress in engineering yeast producers of cellulosic ethanol.FEMS yeast research · 2025Review
- Revealing Non-Conventional and Thermotolerant Yeasts from Tropical Regions: Diversity and Potential Traits for Bioproducts.Mycobiology · 2025Review
- Article
- Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose.FEMS yeast research · 2024Article
- A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts.International journal of molecular sciences · 2023Article
- Metabolic engineering of non-conventional yeasts for construction of the advanced producers of biofuels and high-value chemicals.BBA advances · 2023Article
- The role of hexose transporter-like sensor hxs1 and transcription activator involved in carbohydrate sensing azf1 in xylose and glucose fermentation in the thermotolerant yeast Ogataea polymorpha.Microbial cell factories · 2022Article
- Pathway engineering strategies for improved product yield in yeast-based industrial ethanol production.Synthetic and systems biotechnology · 2022Article
- Respiratory reoxidation of NADH is a key contributor to high oxygen requirements of oxygen-limited cultures of Ogataea parapolymorpha.FEMS yeast research · 2022Article
- Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts.Biotechnology for biofuels · 2021Article
- Screening neutral sites for metabolic engineering of methylotrophic yeastSynthetic and systems biotechnology · 2021Article
- Bioethanolic yeasts from dung beetles: tapping the potential of extremophilic yeasts for improvement of lignocellulolytic feedstock fermentation.Biotechnology for biofuels · 2021Review
- Molecular characterization of Hsf1 as a master regulator of heat shock response in the thermotolerant methylotrophic yeast Ogataea parapolymorpha.Journal of microbiology (Seoul, Korea) · 2021Article
- Engineering of sugar transporters for improvement of xylose utilization during high-temperature alcoholic fermentation in Ogataea polymorpha yeast.Microbial cell factories · 2020Article
- Construction of advanced producers of first- and second-generation ethanol in Saccharomyces cerevisiae and selected species of non-conventional yeasts (Scheffersomyces stipitis, Ogataea polymorpha).Journal of industrial microbiology & biotechnology · 2020Review
- Advances in UsingFrontiers in bioengineering and biotechnology · 2019Review
- Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9/gRNA co-expression plasmid.FEMS yeast research · 2018Article
- Peroxisomes and peroxisomal transketolase and transaldolase enzymes are essential for xylose alcoholic fermentation by the methylotrophic thermotolerant yeast,Biotechnology for biofuels · 2018Article
- Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts.Biotechnology for biofuels · 2018Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe methylotrophic yeast, Hansenula polymorpha is an industrially important microorganism, and belongs to the best studied yeast species with well-developed tools for molecular research. The complete genome sequence of the strain NCYC495 of H. polymorpha is publicly available. Some of the well-studied strains of H. polymorpha are known to ferment glucose, cellobiose and xylose to ethanol at elevated temperature (45 - 50°C) with ethanol yield from xylose significantly lower than that from glucose and cellobiose. Increased yield of ethanol from xylose was demonstrated following directed metabolic changes but, still the final ethanol concentration achieved is well below what is considered feasible for economic recovery by distillation.
resultsIn this work, we describe the construction of strains of H. polymorpha with increased ethanol production from xylose using an ethanol-non-utilizing strain (2EthOH-) as the host. The transformants derived from 2EthOH- overexpressing modified xylose reductase (XYL1m) and native xylitol dehydrogenase (XYL2) were isolated. These transformants produced 1.5-fold more ethanol from xylose than the original host strain. The additional overexpression of XYL3 gene coding for xylulokinase, resulted in further 2.3-fold improvement in ethanol production with no measurable xylitol formed during xylose fermentation. The best ethanol producing strain obtained by metabolic engineering approaches was subjected to selection for resistance to the known inhibitor of glycolysis, the anticancer drug 3-bromopyruvate. The best mutant selected had an ethanol yield of 0.3 g/g xylose and produced up to 9.8 g of ethanol/l during xylose alcoholic fermentation at 45°C without correction for ethanol evaporation.
conclusionsOur results indicate that xylose conversion to ethanol at elevated temperature can be significantly improved in H. polymorpha by combining methods of metabolic engineering and classical selection.
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