ArticleJournal of visualized experiments : JoVE2014
Culturing Caenorhabditis elegans in axenic liquid media and creation of transgenic worms by microparticle bombardment.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- A bacterial enzyme enhances both energy metabolism and health across the life span ofScience advances · 2026Article
- A Robust Chemical-free Platform for Age-SynchronizedbioRxiv : the preprint server for biology · 2026Article
- De novo NADCommunications biology · 2025Article
- The antidiabetic drug metformin aids bacteria in hijacking vitamin B12 from the environment through RcdA.Communications biology · 2023Article
- AxenicJournal of translational autoimmunity · 2020Article
- Measurement of Oxidatively Induced DNA Damage inAnalytical chemistry · 2019Article
- Dual Inhibition ofACS infectious diseases · 2019Article
- Dietary Restriction Extends Lifespan through Metabolic Regulation of Innate Immunity.Cell metabolism · 2019Article
- Iron/Heme Metabolism-Targeted Gallium(III) Nanoparticles Are Active against Extracellular and IntracellularAntimicrobial agents and chemotherapy · 2019Article
- Article
- Agglomeration of Escherichia coli with Positively Charged Nanoparticles Can Lead to Artifacts in a Standard Caenorhabditis elegans Toxicity Assay.Environmental science & technology · 2018Article
- Growth ofG3 (Bethesda, Md.) · 2018Article
- Counting Caenorhabditis elegans: Protocol Optimization and Applications for Population Growth and Toxicity Studies in Liquid Medium.Scientific reports · 2018Article
- A Model for Evolutionary Ecology of Disease: The Case forJournal of nematology · 2017Article
- Environmental influence on Pristionchus pacificus mouth form through different culture methods.Scientific reports · 2017Article
- Metabolic network modeling with model organisms.Current opinion in chemical biology · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
In this protocol, we present the required materials, and the procedure for making modified C. elegans Habituation and Reproduction media (mCeHR). Additionally, the steps for exposing and acclimatizing C. elegans grown on E. coli to axenic liquid media are described. Finally, downstream experiments that utilize axenic C. elegans illustrate the benefits of this procedure. The ability to analyze and determine C. elegans nutrient requirement was illustrated by growing N2 wild type worms in axenic liquid media with varying heme concentrations. This procedure can be replicated with other nutrients to determine the optimal concentration for worm growth and development or, to determine the toxicological effects of drug treatments. The effects of varied heme concentrations on the growth of wild type worms were determined through qualitative microscopic observation and by quantitating the number of worms that grew in each heme concentration. In addition, the effect of varied nutrient concentrations can be assayed by utilizing worms that express fluorescent sensors that respond to changes in the nutrient of interest. Furthermore, a large number of worms were easily produced for the generation of transgenic C. elegans using microparticle bombardment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.