ArticlePLoS genetics2014
Wnt-mediated repression via bipartite DNA recognition by TCF in the Drosophila hematopoietic system.
Article in PLoS genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 42 citations in OpenAlex.
- The E-cadherin-Wnt-mir-994 Axis Repurposes a Cadherin Switch for Niche Robustness and Germline Stem Cell Maintenance.Cell proliferation · 2026Article
- Wnt signaling couples G2 phase control with differentiation during hematopoiesis in Drosophila.Developmental cell · 2024Article
- Wnt target gene activation requires β-catenin separation into biomolecular condensates.PLoS biology · 2024Article
- pop-1/TCF, ref-2/ZIC and T-box factors regulate the development of anterior cells in the C. elegans embryo.Developmental biology · 2022Article
- Preventing erosion of X-chromosome inactivation in human embryonic stem cells.Nature communications · 2022Article
- Intrinsic and Extrinsic Regulation of Hematopoiesis inMolecules and cells · 2022Review
- Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation.Frontiers in cell and developmental biology · 2022Article
- Accessible chromatin reveals regulatory mechanisms underlying cell fate decisions during early embryogenesis.Scientific reports · 2021Article
- β-catenin represses miR455-3p to stimulate m6A modification of HSF1 mRNA and promote its translation in colorectal cancer.Molecular cancer · 2020Article
- Review
- Regulation ofInternational journal of molecular sciences · 2020Review
- Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs.Developmental biology · 2020Article
- The Wnt inhibitor Dkk1 is required for maintaining the normal cardiac differentiation program in Xenopus laevis.Developmental biology · 2019Article
- Review
- Wingless promotes EGFR signaling in follicle stem cells to maintain self-renewal.Development (Cambridge, England) · 2018Article
- Repression of MAP3K1 expression and JNK activity by canonical Wnt signaling.Developmental biology · 2018Article
- Review
- The Wnt Transcriptional Switch: TLE Removal or Inactivation?BioEssays : news and reviews in molecular, cellular and developmental biology · 2018Review
- A C-terminally truncated form of β-catenin acts as a novel regulator of Wnt/β-catenin signaling in planarians.PLoS genetics · 2017Article
- An embryonic system to assess direct and indirect Wnt transcriptional targets.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Wnt/β-catenin signaling pathway plays many important roles in animal development, tissue homeostasis and human disease. Transcription factors of the TCF family mediate many Wnt transcriptional responses, promoting signal-dependent activation or repression of target gene expression. The mechanism of this specificity is poorly understood. Previously, we demonstrated that for activated targets in Drosophila, TCF/Pangolin (the fly TCF) recognizes regulatory DNA through two DNA binding domains, with the High Mobility Group (HMG) domain binding HMG sites and the adjacent C-clamp domain binding Helper sites. Here, we report that TCF/Pangolin utilizes a similar bipartite mechanism to recognize and regulate several Wnt-repressed targets, but through HMG and Helper sites whose sequences are distinct from those found in activated targets. The type of HMG and Helper sites is sufficient to direct activation or repression of Wnt regulated cis-regulatory modules, and protease digestion studies suggest that TCF/Pangolin adopts distinct conformations when bound to either HMG-Helper site pair. This repressive mechanism occurs in the fly lymph gland, the larval hematopoietic organ, where Wnt/β-catenin signaling controls prohemocytic differentiation. Our study provides a paradigm for direct repression of target gene expression by Wnt/β-catenin signaling and allosteric regulation of a transcription factor by DNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.