ArticleNature protocols2014
A simple improved-throughput xylem protoplast system for studying wood formation.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Nitrate transiently rewires transcription in developing xylem of Populus wood to promote cell expansion via CRF4.The Plant cell · 2026Article
- Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production.Nature communications · 2026Article
- Overexpression ofPlants (Basel, Switzerland) · 2026Article
- Advanced technologies of single-cell metabolomics unveiling cellular metabolic heterogeneity for biological and biomedical research.Journal of food and drug analysis · 2026Review
- Genome-wide analysis of the NAC gene family in Qu-1 doubled haploid cell line from Populus simonii × P. nigra and expression patterns under abscisic acid (ABA) treatment.BMC plant biology · 2026Article
- Integrated multi-omics analysis reveals the regulatory mechanism of cryptochrome 1- mediated anthocyanin biosynthesis in Populus trichocarpa.BMC plant biology · 2026Article
- Integrating scRNA-seq and snRNA-seq with spatial transcriptomics to unlock the xylem puzzle.Genome biology · 2026Article
- Integrated morpho-physiological and transcriptomic dissection of waterlogging tolerance inFrontiers in plant science · 2026Article
- The role of PagC3H3 in lignin biosynthesis and insect resistance in Populus alba × Populus glandulosa.Plant cell reports · 2025Article
- Genome-Wide Identification and Characterization of the Growth-Regulating Factor Gene Family Responsive to Abiotic Stresses and Phytohormone Treatments inInternational journal of molecular sciences · 2025Article
- A Protoplast System for CRISPR-Cas Ribonucleoprotein Delivery inPlants (Basel, Switzerland) · 2025Article
- Development of Protoplast-Based Gene Editing System for Areca Palm.Plants (Basel, Switzerland) · 2025Article
- Epigenetic regulation of lignin biosynthesis in wood formation.The New phytologist · 2025Article
- The Class I HD-ZIP transcription factor PagHB7a functions as a positive regulator of salt tolerance inForestry research · 2025Article
- Qu-1: a transformation-and regeneration-amenable doubled haploid cell line with a reference genome sequence for genetic and functional studies inForestry research · 2025Article
- Modern Technologies Provide New Opportunities for Somatic Hybridization in the Breeding of Woody Plants.Plants (Basel, Switzerland) · 2024Review
- Merit of integrating in situ transcriptomics and anatomical information for cell annotation and lineage construction in single-cell analyses of Populus.Genome biology · 2024Article
- Molecular mechanism of salinity and waterlogging tolerance in mangroveFrontiers in plant science · 2024Article
- Comprehensive Analysis of the Catalase (International journal of molecular sciences · 2023Article
- Combining single-cell RNA sequencing with spatial transcriptome analysis reveals dynamic molecular maps of cambium differentiation in the primary and secondary growth of trees.Plant communications · 2023Article
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Authors and funding
13 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolated protoplasts serve as a transient expression system that is highly representative of stable transgenics in terms of transcriptome responses. They can also be used as a cellular system to study gene transactivation and nucleocytoplasmic protein trafficking. They are particularly useful for systems studies in which stable transgenics and mutants are unavailable. We present a protocol for the isolation and transfection of protoplasts from wood-forming tissue, the stem-differentiating xylem (SDX), in the model woody plant Populus trichocarpa. The method involves tissue preparation, digestion of SDX cell walls, protoplast isolation and DNA transfection. Our approach is markedly faster and provides better yields than previous protocols; small (milligrams)- to large (20 g)-scale SDX preparations can be achieved in ~60 s, with isolation of protoplasts and their subsequent transfection taking ~50 min. Up to ten different samples can be processed simultaneously in this time scale. Our protocol gives a high yield (~2.5 × 10(7) protoplasts per g of SDX) of protoplasts sharing 96% transcriptome identity with intact SDX.
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