ArticleMolecular biology of the cell2014
Quantitative analysis and modeling of katanin function in flagellar length control.
Article in Molecular biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- Review
- Role of intraflagellar transport in transcriptional control during flagellar regeneration inMolecular biology of the cell · 2023Article
- The flagellar length control system: exploring the physical biology of organelle size.Physical biology · 2023Review
- Biosynthesis of Linear Protein Nanoarrays Using the Flagellar Axoneme.ACS synthetic biology · 2022Article
- The short flagella 1 (SHF1) gene inMolecular biology of the cell · 2022Article
- PIPCommunications biology · 2022Article
- Article
- Speed and Diffusion of Kinesin-2 Are Competing Limiting Factors in Flagellar Length-Control Model.Biophysical journal · 2020Article
- Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.PLoS biology · 2019Article
- A core mechanism for specifying root vascular patterning can replicate the anatomical variation seen in diverse plant species.Development (Cambridge, England) · 2019Article
- Intraflagellar Transport and Ciliary Dynamics.Cold Spring Harbor perspectives in biology · 2017Review
- KIAA0556 is a novel ciliary basal body component mutated in Joubert syndrome.Genome biology · 2015Article
- How Cells Measure Length on Subcellular Scales.Trends in cell biology · 2015Review
- A NIMA-Related Kinase Suppresses the Flagellar Instability Associated with the Loss of Multiple Axonemal Structures.PLoS genetics · 2015Article
- Microtubule severing by katanin p60 AAA+ ATPase requires the C-terminal acidic tails of both α- and β-tubulins and basic amino acid residues in the AAA+ ring pore.The Journal of biological chemistry · 2015Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Flagellar length control in Chlamydomonas reinhardtii provides a simple model system in which to investigate the general question of how cells regulate organelle size. Previous work demonstrated that Chlamydomonas cytoplasm contains a pool of flagellar precursor proteins sufficient to assemble a half-length flagellum and that assembly of full-length flagella requires synthesis of additional precursors to augment the preexisting pool. The regulatory systems that control the synthesis and regeneration of this pool are not known, although transcriptional regulation clearly plays a role. We used quantitative analysis of length distributions to identify candidate genes controlling pool regeneration and found that a mutation in the p80 regulatory subunit of katanin, encoded by the PF15 gene in Chlamydomonas, alters flagellar length by changing the kinetics of precursor pool utilization. This finding suggests a model in which flagella compete with cytoplasmic microtubules for a fixed pool of tubulin, with katanin-mediated severing allowing easier access to this pool during flagellar assembly. We tested this model using a stochastic simulation that confirms that cytoplasmic microtubules can compete with flagella for a limited tubulin pool, showing that alteration of cytoplasmic microtubule severing could be sufficient to explain the effect of the pf15 mutations on flagellar length.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.