ArticleBMC genomics2014
Genome-wide comparison of microRNAs and their targeted transcripts among leaf, flower and fruit of sweet orange.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 1 of them a synthesis that pooled it.
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Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 88 citations in OpenAlex.
- What Do We Know about Barley miRNAs?International journal of molecular sciences · 2022Pooled it
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- Integration of phytohormones, miRNAs and transcriptome sequencing in cucumber fruit development under high-temperature stress.Food chemistry. Molecular sciences · 2026Article
- Exploring the role of food-source microRNAs as potential nutritional bioactives in humans.NPJ science of food · 2026Article
- MicroRNA-Mediated Hormonal Control of Fruit Morphology.Plants (Basel, Switzerland) · 2026Review
- Sugarcane microRNA shy-miR164 regulates sugar metabolism through direct cleavage of the transcription factor ScNAC mRNA.Plant physiology · 2025Article
- Functional role of microRNA in the regulation of biotic and abiotic stress in agronomic plants.Frontiers in genetics · 2023Review
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- CRISPR/Cas9 mutants of tomato MICRORNA164 genes uncover their functional specialization in development.Plant physiology · 2021Article
- Small RNAs, Degradome, and Transcriptome Sequencing Provide Insights into Papaya Fruit Ripening Regulated by 1-MCP.Foods (Basel, Switzerland) · 2021Article
- Identification and verification of seed development related miRNAs in kernel almond by small RNA sequencing and qPCR.PloS one · 2021Article
- MicroRNA Profiling During Mulberry (Frontiers in plant science · 2021Article
- MicroRNAs in Woody Plants.Frontiers in plant science · 2021Review
- Integrated sRNAome and RNA-Seq analysis reveals miRNA effects on betalain biosynthesis in pitaya.BMC plant biology · 2020Article
- Genome-wide identification and characterization of gibberellin metabolic and signal transduction (GA MST) pathway mediating seed and berry development (SBD) in grape (Vitis vinifera L.).BMC plant biology · 2020Article
- Review
- Abundant expression of maternal siRNAs is a conserved feature of seed development.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-wide analysis of the citrus B3 superfamily and their association with somatic embryogenesis.BMC genomics · 2020Article
- Review
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn plants, microRNAs (miRNAs) regulate gene expression mainly at the post-transcriptional level. Previous studies have demonstrated that miRNA-mediated gene silencing pathways play vital roles in plant development. Here, we used a high-throughput sequencing approach to characterize the miRNAs and their targeted transcripts in the leaf, flower and fruit of sweet orange.
resultsA total of 183 known miRNAs and 38 novel miRNAs were identified. An in-house script was used to identify all potential secondary siRNAs derived from miRNA-targeted transcripts using sRNA and degradome sequencing data. Genome mapping revealed that these miRNAs were evenly distributed across the genome with several small clusters, and 69 pre-miRNAs were co-localized with simple sequence repeats (SSRs). Noticeably, the loop size of pre-miR396c was influenced by the repeat number of CUU unit. The expression pattern of miRNAs among different tissues and developmental stages were further investigated by both qRT-PCR and RNA gel blotting. Interestingly, Csi-miR164 was highly expressed in fruit ripening stage, and was validated to target a NAC transcription factor. This study depicts a global picture of miRNAs and their target genes in the genome of sweet orange, and focused on the comparison among leaf, flower and fruit tissues.
conclusionsThis study provides a global view of miRNAs and their target genes in different tissue of sweet orange, and focused on the identification of miRNA involved in the regulation of fruit ripening. The results of this study lay a foundation for unraveling key regulators of orange fruit development and ripening on post-transcriptional level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.