Evidence map›Paper›PMID 25141858›Full record

ArticleBMC biotechnology2014

Establishment of a reborn MMV-microarray technology: realization of microbiome analysis and other hitherto inaccessible technologies.

Harshita Sharma, Yasunori Kinoshita, Seiichi Fujiu, Shota Nomura, Mizuho Sawada, Shamim Ahmed, Masaki Shibuya, Kosaku Shirai, Syota Takamatsu, Tsuyoshi Watanabe and 11 more

Abstract read
In one paragraph

Article in BMC biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Harshita Sharma
Yasunori Kinoshita
Seiichi Fujiu
Shota Nomura
Mizuho Sawada
Shamim Ahmed
Masaki Shibuya
Kosaku Shirai
Syota Takamatsu
Tsuyoshi Watanabe
Hitoshi Yamazaki
Ryohei Kamiyama
Tetsuya Kobayashi
Hidenao Arai
Miho Suzuki
Naoto Nemoto
Ki Ando
Hidekazu Uchida
Koichiro Kitamura
Osamu Takei
Koichi NishigakiDepartment of Functional Materials Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-okubo, Saitama 338-8570, Japan. koichi@fms.saitama-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWith the accelerating development of bioscience, the problem of research cost has become important. We previously devised and developed a novel concept microarray with manageable volumes (MMV) using a soft gel. It demonstrated the great potential of the MMV technology with the examples of 1024-parallel-cell culture and PCR experiments. However, its full potential failed to be expressed, owing to the nature of the material used for the MMV chip.

resultsIn the present study, by developing plastic-based MMVs and associated technologies, we introduced novel technologies such as C2D2P (in which the cells in each well are converted from DNA to protein in 1024-parallel), NGS-non-dependent microbiome analysis, and other powerful applications.

conclusionsThe reborn MMV-microarray technology has proven to be highly efficient and cost-effective (with approximately 100-fold cost reduction) and enables us to realize hitherto unattainable technologies.

Indexed as

MicrobiotaMicroarray AnalysisPolymerase Chain ReactionReproducibility of Results

Identifiers

PMID25141858
PMCPMC4153446

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.