ArticlePloS one2014
Exploring microRNA-like small RNAs in the filamentous fungus Fusarium oxysporum.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 92 citations in OpenAlex.
- miRNAs as biomarkers for early cancer detection and their application in the development of new diagnostic tools.Biomedical engineering online · 2021Pooled it
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- Trans-Kingdom RNA Dialogues: miRNA and milRNA Networks as Biotechnological Tools for Sustainable Crop Defense and Pathogen Control.Plants (Basel, Switzerland) · 2025Review
- Global discovery, expression pattern, and regulatory role of miRNA-like RNAs inFrontiers in microbiology · 2025Article
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- Network Pharmacology Approaches Used to Identify Therapeutic Molecules for Chronic Venous Disease Based on Potential miRNA Biomarkers.Journal of xenobiotics · 2024Article
- Discovering the hidden function in fungal genomes.Nature communications · 2024Review
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- Epigenetic modulation of fungal pathogens: a focus onFrontiers in microbiology · 2024Review
- Transcriptional dynamics and regulatory function of milRNAs inFrontiers in microbiology · 2024Article
- Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets.BMC genomics · 2023Article
- Extracellular vesicles in phytopathogenic fungi.Extracellular vesicles and circulating nucleic acids · 2023Review
- Comprehensive re-analysis of hairpin small RNAs in fungi reveals loci with conserved links.eLife · 2022Article
- Small RNA Analyses of aViruses · 2022Article
- RNA-Seq, Bioinformatic Identification of Potential MicroRNA-like Small RNAs in the Edible MushroomInternational journal of molecular sciences · 2022Article
- Exploring the Effectiveness and Durability of Trans-Kingdom Silencing of Fungal Genes in the Vascular PathogenInternational journal of molecular sciences · 2022Article
- Discovery of microRNA-like Small RNAs in Pathogenic Plant FungusInternational journal of molecular sciences · 2022Article
- Computational Analysis of HTS Data and Its Application in Plant Pathology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Do small RNAs unlock the below ground microbiome-plant interaction mystery?Frontiers in molecular biosciences · 2022Review
- Cellular and Extracellular Vesicle RNA Analysis in the Global Threat FungusMicrobiology spectrum · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA silencing such as quelling and meiotic silencing by unpaired DNA (MSUD) and several other classes of special small RNAs have been discovered in filamentous fungi recently. More than four different mechanisms of microRNA-like RNAs (milRNAs) production have been illustrated in the model fungus Neurospora crassa including a dicer-independent pathway. To date, very little work focusing on small RNAs in fungi has been reported and no universal or particular characteristic of milRNAs were defined clearly. In this study, small RNA and degradome libraries were constructed and subsequently deep sequenced for investigating milRNAs and their potential cleavage targets on the genome level in the filamentous fungus F. oxysporum f. sp. lycopersici. As a result, there is no intersection of conserved miRNAs found by BLASTing against the miRBase. Further analysis showed that the small RNA population of F. oxysporum shared many common features with the small RNAs from N. crassa and other fungi. According to the known standards of miRNA prediction in plants and animals, milRNA candidates from 8 families (comprising 19 members) were screened out and identified. However, none of them could trigger target cleavage based on the degradome data. Moreover, most major signals of cleavage in transcripts could not match appropriate complementary small RNAs, suggesting that other predominant modes for milRNA-mediated gene regulation could exist in F. oxysporum. In addition, the PAREsnip program was utilized for comprehensive analysis and 3 families of small RNAs leading to transcript cleavage were experimentally validated. Altogether, our findings provided valuable information and important hints for better understanding the functions of the small RNAs and milRNAs in the fungal kingdom.
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Registered trials
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