ArticlePloS one2014
Species-specific detection and identification of fusarium species complex, the causal agent of sugarcane pokkah boeng in China.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Fusarium sacchari 14-3-3 Protein FsBmh1 Sequesters a Novel Elicitor FsEcm33 to Evade Host Immunity.Molecular plant pathology · 2026Article
- Bifunctional trehalase FsTreA coordinates intracellular mobilization and extracellular utilization of trehalose to modulate virulence inApplied and environmental microbiology · 2026Article
- A Fusarium sacchari Glycoside Hydrolase 12 Protein FsEG1 Is a Major Virulence Factor During Sugarcane Infection and Confers Resistance to Pokkah Boeng Disease via the HIGS Strategy.Plant biotechnology journal · 2026Article
- Secretome-Wide Lysine Acetylation Profiling ofMicroorganisms · 2026Article
- Characterization and Genome Analysis ofMicroorganisms · 2025Article
- Genome-Wide Identification and Functional Characterization of the Acyl-CoA Dehydrogenase (ACAD) Family inInternational journal of molecular sciences · 2025Article
- Mining of Candidate Genes and Developing Molecular Markers Associated with Pokkah Boeng Resistance in Sugarcane (Plants (Basel, Switzerland) · 2024Article
- Article
- Fusarium sacchari hypovirus 1, a Member ofViruses · 2024Article
- Redundant and Distinct Roles of Two 14-3-3 Proteins inJournal of fungi (Basel, Switzerland) · 2024Article
- FsCGBP, a Cutinase G-Box Binding Protein, Regulates the Growth, Development, and Virulence ofJournal of fungi (Basel, Switzerland) · 2024Article
- Development of a PCR-based assay for specific and sensitive detection of Fusarium buharicum from infected okra plant.PloS one · 2024Article
- Integration of Transcriptomic and Metabolomic Profiles Provides Insights into the Influence of Nitrogen on Secondary Metabolism inInternational journal of molecular sciences · 2023Article
- Pectate Lyase from Fusarium sacchari Induces Plant Immune Responses and Contributes to Virulence.Microbiology spectrum · 2023Article
- Predication of the Effector Proteins Secreted byJournal of fungi (Basel, Switzerland) · 2022Article
- Pineapple Mycobiome Related to Fruitlet Core Rot Occurrence and the Influence of Fungal Species Dispersion Patterns.Journal of fungi (Basel, Switzerland) · 2021Article
- Determination of Diversity, Distribution and Host Specificity of KoreanMycobiology · 2021Article
- Molecular Identification of Secreted Effector Genes Involved in AfricanFrontiers in cellular and infection microbiology · 2020Article
- Article
- Virome Identification and Characterization ofFrontiers in microbiology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPokkah boeng disease caused by the Fusarium species complex results in significant yield losses in sugarcane. Thus, the rapid and accurate detection and identification of the pathogen is urgently required to manage and prevent the spreading of sugarcane pokkah boeng.
methodsA total of 101 isolates were recovered from the pokkah boeng samples collected from five major sugarcane production areas in China throughout 2012 and 2013. The causal pathogen was identified by morphological observation, pathogenicity test, and phylogenetic analysis based on the fungus-conserved rDNA-ITS. Species-specific TaqMan real-time PCR and conventional PCR methods were developed for rapid and accurate detection of the causal agent of sugarcane pokkah boeng. The specificity and sensitivity of PCR assay were also evaluated on a total of 84 isolates of Fusarium from China and several isolates from other fungal pathogens of Sporisorium scitamineum and Phoma sp. and sugarcane endophyte of Acremonium sp.
resultTwo Fusarium species (F. verticillioides and F. proliferatum) that caused sugarcane pokahh boeng were identified by morphological observation, pathogenicity test, and phylogenetic analysis. Species-specific TaqMan PCR and conventional PCR were designed and optimized to target their rDNA-ITS regions. The sensitivity of the TaqMan PCR was approximately 10 pg of fungal DNA input, which was 1,000-fold over conventional PCR, and successfully detected pokkah boeng in the field-grown sugarcane. CONCLUSIONS/SIGNIFICANCE: This study was the first to identify two species, F. verticillioides and F. proliferatum, that were causal pathogens of sugarcane pokkah boeng in China. It also described the development of a species-specific PCR assay to detect and confirm these pathogens in sugarcane plants from mainland China. This method will be very useful for a broad range of research endeavors as well as the regulatory response and management of sugarcane pokkah boeng.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.