ArticlePloS one2014
A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Early Cell Adhesion of Periodontal Ligament Stem Cells on 3D Printed Polylactic Acid/Hydroxyapatite Scaffolds: An In Vitro Study.Polymers · 2025Article
- Multifunctional electrospun nanofibrous scaffold enriched with alendronate and hydroxyapatite for balancing osteogenic and osteoclast activity to promote bone regeneration.Frontiers in bioengineering and biotechnology · 2023Article
- Electrospun Biomimetic Nanofibrous Scaffolds: A Promising Prospect for Bone Tissue Engineering and Regenerative Medicine.International journal of molecular sciences · 2022Review
- Effects of nanofiber scaffolds coated with nanoparticulate and micro-particulate FDBA on the morphology, adhesion and proliferation of human mesenchymal stem cells.Iranian biomedical journal · 2022Article
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- Polydopamine-modified poly(l-lactic acid) nanofiber scaffolds immobilized with an osteogenic growth peptide for bone tissue regeneration.RSC advances · 2019Article
- The physicochemical and biomechanical profile of forsterite and its osteogenic potential of mesenchymal stromal cells.PloS one · 2019Article
- In vitro response of dental pulp stem cells in 3D scaffolds: A regenerative bone material.Heliyon · 2018Article
- Osteogenic differentiation of mesenchymal stem cells cultured on PLLA scaffold coated with Wharton's Jelly.EXCLI journal · 2017Article
- Incorporation of Fucoidan in β-Tricalcium phosphate-Chitosan scaffold prompts the differentiation of human bone marrow stromal cells into osteogenic lineage.Scientific reports · 2016Article
- Application of Collagen Scaffold in Tissue Engineering: Recent Advances and New Perspectives.Polymers · 2016Review
- An update on the Application of Nanotechnology in Bone Tissue Engineering.The open orthopaedics journal · 2016Article
- Nanotechnology in bone tissue engineering.Nanomedicine : nanotechnology, biology, and medicine · 2015Review
- Taking cues from the extracellular matrix to design bone-mimetic regenerative scaffolds.Matrix biology : journal of the International Society for Matrix BiologyReview
Corrections and comments
- Erratum issuedRaman Murali, Malliga [corrected to Murali, Malliga R]; Vasudevaraj Naveen, Sangeetha [corrected to Naveen, Sangeetha V]
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs) differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200-950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (P<0.05) observed at different time points (0, 7, 14, and 21 days). Furthermore, electron microscopic observations and fibronectin localization revealed that PLLA/Col/HA scaffolds exhibited superior osteoinductivity, when compared with PLLA/Col or PLLA/HA scaffolds. These findings indicated that the fibrous structure and synergistic action of Col and nano-HA with high-molecular-weight PLLA played a vital role in inducing osteogenic differentiation of hMSCs. The data obtained in this study demonstrated that the developed fibrous PLLA/Col/HA biocomposite scaffold may be supportive for stem cell based therapies for bone repair, when compared with the other two scaffolds.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.