ArticlePloS one2014
Oral delivery mediated RNA interference of a carboxylesterase gene results in reduced resistance to organophosphorus insecticides in the cotton Aphid, Aphis gossypii Glover.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 109 citations in OpenAlex.
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- Identification of lncRNAs Associated with Imidacloprid Resistance in the Grain Aphid Rhopalosiphum padi (L.) (Hemiptera: Aphididae).Neotropical entomology · 2025Article
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- ApCarE4 and ApPOD3 participate in the adaptation of pea aphids to different alfalfa varieties.Scientific reports · 2024Article
- Insights into the Effects of Insecticides on Aphids (Hemiptera: Aphididae): Resistance Mechanisms and Molecular Basis.International journal of molecular sciences · 2023Review
- Cross-protection interactions in insect pests: Implications for pest management in a changing climate.Pest management science · 2023Review
- Expression of Modified Snowdrop Lectin (Genes · 2022Article
- RNA Interference for Improving Disease Resistance in Plants and Its Relevance in This Clustered Regularly Interspaced Short Palindromic Repeats-Dominated Era in Terms of dsRNA-Based Biopesticides.Frontiers in plant science · 2022Review
- Double-Strand RNA (dsRNA) Delivery Methods in Insects: Diaphorina citri.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- RNAi-Based Functional Genomics in Hemiptera.Insects · 2020Review
- The Roles of DNA Methyltransferases 1 (DNMT1) in Regulating Sexual Dimorphism in the Cotton Mealybug,Insects · 2020Article
- A Conserved Carboxylesterase InhibitsViruses · 2020Article
- Insecticidal Gene Silencing by RNAi in the Neotropical Region.Neotropical entomology · 2020Review
- An RNAi supplemented diet as a reverse genetics tool to control bluegreen aphid, a major pest of legumes.Scientific reports · 2020Article
- Bt, Not a Threat toFrontiers in physiology · 2020Article
- Function Analysis of P450 and GST Genes to Imidacloprid inFrontiers in physiology · 2020Article
- Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method inFrontiers in physiology · 2020Article
- Transcription Factors AhR/ARNT Regulate the Expression ofInternational journal of molecular sciences · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference (RNAi) is an effective tool to examine the function of individual genes. Carboxylesterases (CarE, EC 3.1.1.1) are known to play significant roles in the metabolism of xenobiotic compounds in many insect species. Previous studies in our laboratory found that CarE expression was up-regulated in Aphis gossypii (Glover) (Hemiptera: Aphididae) adults of both omethoate and malathion resistant strains, indicating the potential involvement of CarE in organophosphorus (OP) insecticide resistance. Functional analysis (RNAi) is therefore warranted to investigate the role of CarE in A. gossypii to OPs resistance.
resultCarE expression in omethoate resistant individuals of Aphis gossypii was dramatically suppressed following ingestion of dsRNA-CarE. The highest knockdown efficiency (33%) was observed at 72 h after feeding when dsRNA-CarE concentration was 100 ng/µL. The CarE activities from the CarE knockdown aphids were consistent with the correspondingly significant reduction in CarE expression. The CarE activity in the individuals of control aphids was concentrated in the range of 650-900 mOD/per/min, while in the individuals of dsRNA-CarE-fed aphids, the CarE activity was concentrated in the range of 500-800 mOD/per/min. In vitro inhibition experiments also demonstrated that total CarE activity in the CarE knockdown aphids decreased significantly as compared to control aphids. Bioassay results of aphids fed dsRNA-CarE indicated that suppression of CarE expression increased susceptibility to omethoate in individuals of the resistant aphid strains.
conclusionThe results of this study not only suggest that ingestion of dsRNA through artificial diet could be exploited for functional genomic studies in cotton aphids, but also indicate that CarE can be considered as a major target of organophosphorus insecticide (OPs) resistance in A. gossypii. Further, our results suggest that the CarE would be a propitious target for OPs resistant aphid control, and insect-resistant transgenic plants may be obtained through plant RNAi-mediated silencing of insect CarE expression.
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