ArticleBiophysical journal2014
Quantitation of malaria parasite-erythrocyte cell-cell interactions using optical tweezers.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Review
- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- Application of optical tweezer technology reveals that PfEBA and PfRH ligands, not PfMSP1, play a central role in Plasmodium falciparum merozoite-erythrocyte attachment.PLoS pathogens · 2024Article
- Effective cell membrane tension protects red blood cells against malaria invasion.PLoS computational biology · 2023Article
- Optical trapping with holographically structured light for single-cell studies.Biophysics reviews · 2023Review
- Directed assembly of genetically engineered eukaryotic cells into living functional materials via ultrahigh-affinity protein interactions.Science advances · 2022Article
- A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers.Biosensors · 2022Article
- The erythrocyte membrane properties of beta thalassaemia heterozygotes and their consequences for Plasmodium falciparum invasion.Scientific reports · 2022Article
- Biophysical Tools and Concepts Enable Understanding of Asexual Blood Stage Malaria.Frontiers in cellular and infection microbiology · 2022Review
- Protein Unfolding: Denaturant vs. Force.Biomedicines · 2021Article
- Model systems for optical trapping: the physical basis and biological applications.Biophysical reviews · 2021Review
- Article
- Full-lengtheLife · 2020Article
- Recent Advances on the Model, Measurement Technique, and Application of Single Cell Mechanics.International journal of molecular sciences · 2020Review
- Stochastic bond dynamics facilitates alignment of malaria parasite at erythrocyte membrane upon invasion.eLife · 2020Article
- Induction and measurement of the early stage of a host-parasite interaction using a combined optical trapping and Raman microspectroscopy system.Journal of biophotonics · 2020Article
- Importance of Erythrocyte Deformability for the Alignment of Malaria Parasite upon Invasion.Biophysical journal · 2019Article
- Plasmodium myosin A drives parasite invasion by an atypical force generating mechanism.Nature communications · 2019Article
- A pan-apicomplexan phosphoinositide-binding protein acts in malarial microneme exocytosis.EMBO reports · 2019Article
- Microfluidic Analysis for Separating and Measuring the Deformability of Cancer Cell Subpopulations.ACS omega · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Erythrocyte invasion by Plasmodium falciparum merozoites is an essential step for parasite survival and hence the pathogenesis of malaria. Invasion has been studied intensively, but our cellular understanding has been limited by the fact that it occurs very rapidly: invasion is generally complete within 1 min, and shortly thereafter the merozoites, at least in in vitro culture, lose their invasive capacity. The rapid nature of the process, and hence the narrow time window in which measurements can be taken, have limited the tools available to quantitate invasion. Here we employ optical tweezers to study individual invasion events for what we believe is the first time, showing that newly released P. falciparum merozoites, delivered via optical tweezers to a target erythrocyte, retain their ability to invade. Even spent merozoites, which had lost the ability to invade, retain the ability to adhere to erythrocytes, and furthermore can still induce transient local membrane deformations in the erythrocyte membrane. We use this technology to measure the strength of the adhesive force between merozoites and erythrocytes, and to probe the cellular mode of action of known invasion inhibitory treatments. These data add to our understanding of the erythrocyte-merozoite interactions that occur during invasion, and demonstrate the power of optical tweezers technologies in unraveling the blood-stage biology of malaria.
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