ArticleBiophysical journal2014
Single-molecule tracking of inositol trisphosphate receptors reveals different motilities and distributions.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
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- Visualizing PIEZO1 Localization and Activity in hiPSC-Derived Single Cells and Organoids with HaloTag Technology.bioRxiv : the preprint server for biology · 2025Article
- Capture at the ER-mitochondrial contacts licenses IPNature communications · 2022Article
- KRAP is required for diffuse and punctate IPCell calcium · 2022Article
- KRAP tethers IPNature communications · 2021Article
- IPeLife · 2020Article
- Spatial-temporal patterning of CaSeminars in cell & developmental biology · 2019Review
- Simulation of calcium signaling in fine astrocytic processes: Effect of spatial properties on spontaneous activity.PLoS computational biology · 2019Article
- Structure and Function of IPCold Spring Harbor perspectives in biology · 2019Review
- Myosin-II mediated traction forces evoke localized Piezo1-dependent CaCommunications biology · 2019Article
- All three IPScience signaling · 2018Article
- Dynamic CaCell calcium · 2018Article
- CaNature communications · 2017Article
- IPCellular and molecular life sciences : CMLS · 2017Review
- Hindered cytoplasmic diffusion of inositol trisphosphate restricts its cellular range of action.Science signaling · 2016Article
- Mode switching of Inositol 1,4,5-trisphosphate receptor channel shapes the Spatiotemporal scales of CaJournal of biological physics · 2016Article
- Inositol 1,4,5-trisphosphate receptors and their protein partners as signalling hubs.The Journal of physiology · 2016Review
- On the Adjacency Matrix of RyR2 Cluster Structures.PLoS computational biology · 2015Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Puffs are local Ca(2+) signals that arise by Ca(2+) liberation from the endoplasmic reticulum through the concerted opening of tightly clustered inositol trisphosphate receptors/channels (IP3Rs). The locations of puff sites observed by Ca(2+) imaging remain static over several minutes, whereas fluorescence recovery after photobleaching (FRAP) experiments employing overexpression of fluorescently tagged IP3Rs have shown that the majority of IP3Rs are freely motile. To address this discrepancy, we applied single-molecule imaging to locate and track type 1 IP3Rs tagged with a photoswitchable fluorescent protein and expressed in COS-7 cells. We found that ∼ 70% of the IP3R1 molecules were freely motile, undergoing random walk motility with an apparent diffusion coefficient of ∼ 0.095 μm s(-1), whereas the remaining molecules were essentially immotile. A fraction of the immotile IP3Rs were organized in clusters, with dimensions (a few hundred nanometers across) comparable to those previously estimated for the IP3R clusters underlying functional puff sites. No short-term (seconds) changes in overall motility or in clustering of immotile IP3Rs were apparent following activation of IP3/Ca(2+) signaling. We conclude that stable clusters of small numbers of immotile IP3Rs may underlie local Ca(2+) release sites, whereas the more numerous motile IP3Rs appear to be functionally silent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.