ArticleAnalytical chemistry2014
¹³C NMR metabolomics: applications at natural abundance.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Article
- PyINETA: Open-Source Platform for INADEQUATE-JRES Integration in NMR Metabolomics.Analytical chemistry · 2024Article
- PyINETA: Open-source platform for INADEQUATE-JRES integration in NMR metabolomics.bioRxiv : the preprint server for biology · 2024Article
- Comparison of the Metabolic Profile between Unstimulated and Stimulated Saliva Samples from Pregnant Women with/without Obesity and Periodontitis.Journal of personalized medicine · 2023Article
- Article
- Metabolic rewiring in MYC-driven medulloblastoma by BET-bromodomain inhibition.Scientific reports · 2023Article
- Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (Metabolites · 2022Article
- Application of Counter-wound Multi-arm Spirals in HTS Resonator Design.IEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee · 2022Article
- Article
- Metabolomics on the study of marine organisms.Metabolomics : Official journal of the Metabolomic Society · 2022Review
- Implementing High Q-Factor HTS Resonators to Enhance Probe Sensitivity inJournal of physics. Conference series · 2022Article
- Fluxomics - New Metabolomics Approaches to Monitor Metabolic Pathways.Frontiers in pharmacology · 2022Review
- Fine optimization of a dissolution dynamic nuclear polarization experimental setting forMagnetic resonance (Gottingen, Germany) · 2022Article
- Extending the Scope ofAnalytical chemistry · 2021Article
- Progress Towards a Higher SensitivityIEEE transactions on applied superconductivity : a publication of the IEEE Superconductivity Committee · 2021Article
- Nuclear Magnetic Resonance Spectroscopy in Clinical Metabolomics and Personalized Medicine: Current Challenges and Perspectives.Frontiers in molecular biosciences · 2021Review
- Hyperpolarized NMR Metabolomics at NaturalAnalytical chemistry · 2020Article
- Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures.International journal of molecular sciences · 2020Review
- Phosphorus NMR and Its Application to Metabolomics.Analytical chemistry · 2020Article
- NMR Spectroscopy-Based Metabolic Profiling of Biospecimens.Current protocols in protein science · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
(13)C NMR has many advantages for a metabolomics study, including a large spectral dispersion, narrow singlets at natural abundance, and a direct measure of the backbone structures of metabolites. However, it has not had widespread use because of its relatively low sensitivity compounded by low natural abundance. Here we demonstrate the utility of high-quality (13)C NMR spectra obtained using a custom (13)C-optimized probe on metabolomic mixtures. A workflow was developed to use statistical correlations between replicate 1D (13)C and (1)H spectra, leading to composite spin systems that can be used to search publicly available databases for compound identification. This was developed using synthetic mixtures and then applied to two biological samples, Drosophila melanogaster extracts and mouse serum. Using the synthetic mixtures we were able to obtain useful (13)C-(13)C statistical correlations from metabolites with as little as 60 nmol of material. The lower limit of (13)C NMR detection under our experimental conditions is approximately 40 nmol, slightly lower than the requirement for statistical analysis. The (13)C and (1)H data together led to 15 matches in the database compared to just 7 using (1)H alone, and the (13)C correlated peak lists had far fewer false positives than the (1)H generated lists. In addition, the (13)C 1D data provided improved metabolite identification and separation of biologically distinct groups using multivariate statistical analysis in the D. melanogaster extracts and mouse serum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.