ArticleBioMed research international2014
Cytoskeleton modifications and autophagy induction in TCam-2 seminoma cells exposed to simulated microgravity.
Article in BioMed research international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Impact of Microgravity on Cytoskeletal Dynamics, Protein Transport, and Signaling Networks: Potential Therapeutic Opportunities for Skin Health.International journal of molecular sciences · 2026Review
- Microgravity and cancer cells: from molecular mechanisms to therapeutic strategies.Cell death discovery · 2026Review
- Cytoskeleton-mediated autophagy regulation in neuroimmune contexts: molecular mechanisms and functional perspectives.Frontiers in immunology · 2026Review
- The Cytoskeleton in Space.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Oxidative Damage Under Microgravity Conditions: Response Mechanisms, Monitoring Methods and Countermeasures on Somatic and Germ Cells.International journal of molecular sciences · 2025Review
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- Influence of Simulated Microgravity on Mammary Epithelial Cells Grown as 2D and 3D Cultures.International journal of molecular sciences · 2023Article
- RCCS Bioreactor-Based Modeled Microgravity Affects Gastric Cancer Cells and Improves the Chemotherapeutic Effect.Membranes · 2022Article
- Cyclic mechanical strain with high-tensile triggers autophagy in growth plate chondrocytes.Journal of orthopaedic surgery and research · 2022Article
- Spaceflight affects neuronal morphology and alters transcellular degradation of neuronal debris in adultiScience · 2021Article
- Role of Apoptosis in Wound Healing and Apoptosis Alterations in Microgravity.Frontiers in bioengineering and biotechnology · 2021Review
- Implications of Altered Endosome and Lysosome Biology in Space Environments.International journal of molecular sciences · 2020Review
- Effects of Simulated Microgravity on Ultrastructure and Apoptosis of Choroidal Vascular Endothelial Cells.Frontiers in physiology · 2020Article
- Autophagy protects HUVECs against ER stress-mediated apoptosis under simulated microgravity.Apoptosis : an international journal on programmed cell death · 2019Article
- Physiological Responses of Jurkat Lymphocytes to Simulated Microgravity Conditions.International journal of molecular sciences · 2019Article
- Transient increases in intracellular calcium and reactive oxygen species levels in TCam-2 cells exposed to microgravity.Scientific reports · 2017Article
- The influence of simulated microgravity on proliferation and apoptosis in U251 glioma cells.In vitro cellular & developmental biology. Animal · 2017Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of how mechanical forces may influence cell behavior via cytoskeleton remodeling is a relevant challenge of nowadays that may allow us to define the relationship between mechanics and biochemistry and to address the larger problem of biological complexity. An increasing amount of literature data reported that microgravity condition alters cell architecture as a consequence of cytoskeleton structure modifications. Herein, we are reporting the morphological, cytoskeletal, and behavioral modifications due to the exposition of a seminoma cell line (TCam-2) to simulated microgravity. Even if no differences in cell proliferation and apoptosis were observed after 24 hours of exposure to simulated microgravity, scanning electron microscopy (SEM) analysis revealed that the change of gravity vector significantly affects TCam-2 cell surface morphological appearance. Consistent with this observation, we found that microtubule orientation is altered by microgravity. Moreover, the confocal analysis of actin microfilaments revealed an increase in the cell width induced by the low gravitational force. Microtubules and microfilaments have been related to autophagy modulation and, interestingly, we found a significant autophagic induction in TCam-2 cells exposed to simulated microgravity. This observation is of relevant interest because it shows, for the first time, TCam-2 cell autophagy as a biological response induced by a mechanical stimulus instead of a biochemical one.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.