ArticleFrontiers in microbiology2014
Grappling archaea: ultrastructural analyses of an uncultivated, cold-loving archaeon, and its biofilm.
Article in Frontiers in microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 32 citations in OpenAlex.
- Extracellular Vesicles in Bacteria, Archaea, and Eukaryotes: Mechanisms of Inter-Kingdom Communication and Clinical Implications.Microorganisms · 2025Review
- First Insights into the Bacterial Diversity of Mount Etna Volcanic Caves.Microbial ecology · 2023Article
- Archaeosomes: New Generation of Liposomes Based on Archaeal Lipids for Drug Delivery and Biomedical Applications.ACS omega · 2023Review
- The cell biology of archaea.Nature microbiology · 2022Review
- Taxonomic and functional analyses of intact microbial communities thriving in extreme, astrobiology-relevant, anoxic sites.Microbiome · 2021Article
- Archaeal biofilm formation.Nature reviews. Microbiology · 2018Review
- Adhesion to nanofibers drives cell membrane remodeling through one-dimensional wetting.Nature communications · 2018Article
- Archaeal cell surface biogenesis.FEMS microbiology reviews · 2018Article
- Electron microscopic observations of prokaryotic surface appendages.Journal of microbiology (Seoul, Korea) · 2017Review
- Structure andeLife · 2017Article
- Novel Large Sulfur Bacteria in the Metagenomes of Groundwater-Fed Chemosynthetic Microbial Mats in the Lake Huron Basin.Frontiers in microbiology · 2017Article
- Computational Exploration of Putative LuxR Solos in Archaea and Their Functional Implications in Quorum Sensing.Frontiers in microbiology · 2017Article
- Culture Independent Genomic Comparisons Reveal Environmental Adaptations for Altiarchaeales.Frontiers in microbiology · 2016Article
- "Altiarchaeales": uncultivated archaea from the subsurface.Life (Basel, Switzerland) · 2015Review
- S-layers at second glance? Altiarchaeal grappling hooks (hami) resemble archaeal S-layer proteins in structure and sequence.Frontiers in microbiology · 2015Article
- Archaeal type IV pili and their involvement in biofilm formation.Frontiers in microbiology · 2015Article
- Cytochromes c in Archaea: distribution, maturation, cell architecture, and the special case of Ignicoccus hospitalis.Frontiers in microbiology · 2015Article
- Getting a hold on archaeal type IV pili: an expanding repertoire of cellular appendages implicates complex regulation and diverse functions.Frontiers in microbiology · 2015Article
- Intra- and inter-species interactions in microbial communities.Frontiers in microbiology · 2014Article
- S-layer and cytoplasmic membrane - exceptions from the typical archaeal cell wall with a focus on double membranes.Frontiers in microbiology · 2014Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Similarly to Bacteria, Archaea are microorganisms that interact with their surrounding environment in a versatile manner. To date, interactions based on cellular structure and surface appendages have mainly been documented using model systems of cultivable archaea under laboratory conditions. Here, we report on the microbial interactions and ultrastructural features of the uncultivated SM1 Euryarchaeon, which is highly dominant in its biotope. Therefore, biofilm samples taken from the Sippenauer Moor, Germany, were investigated via transmission electron microscopy (TEM; negative staining, thin-sectioning) and scanning electron microscopy (SEM) in order to elucidate the fine structures of the microbial cells and the biofilm itself. The biofilm consisted of small archaeal cocci (0.6 μm diameter), arranged in a regular pattern (1.0-2.0 μm distance from cell to cell), whereas each archaeon was connected to 6 other archaea on average. Extracellular polymeric substances (EPS) were limited to the close vicinity of the archaeal cells, and specific cell surface appendages (hami, Moissl et al., 2005) protruded beyond the EPS matrix enabling microbial interaction by cell-cell contacts among the archaea and between archaea and bacteria. All analyzed hami revealed their previously described architecture of nano-grappling hooks and barb-wire basal structures. Considering the archaeal cell walls, the SM1 Euryarchaea exhibited a double-membrane, which has rarely been reported for members of this phylogenetic domain. Based on these findings, the current generalized picture on archaeal cell walls needs to be revisited, as archaeal cell structures are more complex and sophisticated than previously assumed, particularly when looking into the uncultivated majority.
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