ArticleProceedings of the National Academy of Sciences of the United States of America2014
Immunolocalization of skeletal matrix proteins in tissue and mineral of the coral Stylophora pistillata.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 103 citations in OpenAlex.
- Lightweight Carbon From Lightweight Biomass: A Nature-Inspired Carbon Nexus for Smart Applications.Chemical record (New York, N.Y.) · 2026Review
- Coral growth, retraction, defense, and regenerative strategies revealed by live microCT.Science advances · 2026Article
- Building like a Coral-Parallelized, Multiscale Biofabrication.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- 4D Insights into Coral Biomineralization: Effects of Ocean Acidification on the Early Skeleton Development of a Stony Coral.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Enhancing Osteogenesis through Bio-Inspired Recombinant Coral Protein Galaxin by Targeting Mitochondrial Metabolism and ATP Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A noveliScience · 2024Article
- The response of coral skeletal nano structure and hardness to ocean acidification conditions.Royal Society open science · 2023Article
- Organic Controls over Biomineral Ca-Mg Carbonate Compositions and Morphologies.Crystal growth & design · 2023Article
- Different skeletal protein toolkits achieve similar structure and performance in the tropical coral Stylophora pistillata and the temperate Oculina patagonica.Scientific reports · 2022Article
- Environmental memory gained from exposure to extreme pCOProceedings. Biological sciences · 2022Article
- Investigating calcification-related candidates in a non-symbiotic scleractinian coral, Tubastraea spp.Scientific reports · 2022Article
- Multiscale mechanical consequences of ocean acidification for cold-water corals.Scientific reports · 2022Article
- Sclerites of the soft coral Ovabunda macrospiculata (Xeniidae) are predominantly the metastable CaCOActa biomaterialia · 2021Article
- The Evolution of Calcification in Reef-Building Corals.Molecular biology and evolution · 2021Article
- Phase-Field Modeling of Biomineralization in Mollusks and Corals: Microstructure vs Formation Mechanism.JACS Au · 2021Article
- Combined responses of primary coral polyps and their algal endosymbionts to decreasing seawater pH.Proceedings. Biological sciences · 2021Article
- The spatial network of skeletal proteins in a stony coral.Journal of the Royal Society, Interface · 2021Article
- Crystal nucleation and growth of spherulites demonstrated by coral skeletons and phase-field simulations.Acta biomaterialia · 2021Article
- First sequencing of ancient coral skeletal proteins.Scientific reports · 2020Article
- The role of aspartic acid in reducing coral calcification under ocean acidification conditions.Scientific reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precipitation and assembly of calcium carbonate skeletons by stony corals is a precisely controlled process regulated by the secretion of an ECM. Recently, it has been reported that the proteome of the skeletal organic matrix (SOM) contains a group of coral acid-rich proteins as well as an assemblage of adhesion and structural proteins, which together, create a framework for the precipitation of aragonite. To date, we are aware of no report that has investigated the localization of individual SOM proteins in the skeleton. In particular, no data are available on the ultrastructural mapping of these proteins in the calcification site or the skeleton. This information is crucial to assessing the role of these proteins in biomineralization. Immunological techniques represent a valuable approach to localize a single component within a calcified skeleton. By using immunogold labeling and immunohistochemical assays, here we show the spatial arrangement of key matrix proteins in tissue and skeleton of the common zooxanthellate coral, Stylophora pistillata. To our knowledge, our results reveal for the first time that, at the nanoscale, skeletal proteins are embedded within the aragonite crystals in a highly ordered arrangement consistent with a diel calcification pattern. In the tissue, these proteins are not restricted to the calcifying epithelium, suggesting that they also play other roles in the coral's metabolic pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.