Evidence map›Paper›PMID 25139361›Full record

ArticleJournal of dental research2014

Fluorapatite-modified scaffold on dental pulp stem cell mineralization.

T Guo, Y Li, G Cao, Z Zhang, S Chang, A Czajka-Jakubowska, J E Nör, B H Clarkson, J Liu

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of dental research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

T GuoDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA Department of Stomatology, Nanjing Jinling Hospital, Nanjing, Jiangsu, China.
Y LiDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA Department of Oral and Maxillofacial Surgery, State Key Laboratory of Military Stomatology, School of Stomatology, The Fourth Military Medical University, Xian, Shaanxi, China.
G CaoDepartment of Stomatology, Nanjing Jinling Hospital, Nanjing, Jiangsu, China.
Z ZhangDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA.
S ChangDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA.
A Czajka-JakubowskaDepartment of Maxillofacial Orthopedics and Orthodontics, Poznan University of Medical Sciences, Poznan, Poland.
J E NörDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA.
B H ClarksonDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA.
J LiuDepartment of Cariology, Restorative Sciences and Endodontics, Dental School, University of Michigan, Ann Arbor, MI, USA junlc@umich.edu.
University of Michigan–Ann Arbor · USJinling Institute of Technology · CNAir Force Medical University · CNPoznan University of Medical Sciences · PL

Funding

Mechanisms of dental pulp stem cell differentiation into functional endotheliumR01DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI NOR, JACQUES EDUARDO · 2011 to 2021
$3.2M
Regulation of dental pulp stem cell fateR37DE021410 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jacques Eduardo Nor · 2024 to 2026
$1.2M
NIDCR NIH HHS R37 DE021410
6 · The paper itself

Abstract

In previous studies, fluorapatite (FA) crystal-coated surfaces have been shown to stimulate the differentiation and mineralization of human dental pulp stem cells (DPSCs) in two-dimensional cell culture. However, whether the FA surface can recapitulate these properties in three-dimensional culture is still unknown. This study examined the differences in behavior of human DPSCs cultured on electrospun polycaprolactone (PCL) NanoECM nanofibers with or without the FA crystals. Under near-physiologic conditions, the FA crystals were synthesized on the PCL nanofiber scaffolds. The FA crystals were evenly distributed on the scaffolds. DPSCs were cultured on the PCL+FA or the PCL scaffolds for up to 28 days. Scanning electron microscope images showed that DPSCs attached well to both scaffolds after the initial seeding. However, it appeared that more multicellular aggregates formed on the PCL+FA scaffolds. After 14 days, the cell proliferation on the PCL+FA was slower than that on the PCL-only scaffolds. Interestingly, even without any induction of mineralization, from day 7, the upregulation of several pro-osteogenic molecules (dmp1, dspp, runx2, ocn, spp1, col1a1) was detected in cells seeded on the PCL+FA scaffolds. A significant increase in alkaline phosphatase activity was also seen on FA-coated scaffolds compared with the PCL-only scaffolds at days 14 and 21. At the protein level, osteocalcin expression was induced only in the DPSCs on the PCL+FA surfaces at day 21 and then significantly enhanced at day 28. A similar pattern was observed in those specimens stained with Alizarin red and Von Kossa after 21 and 28 days. These data suggest that the incorporation of FA crystals within the three-dimensional PCL nanofiber scaffolds provided a favorable extracellular matrix microenvironment for the growth, differentiation, and mineralization of human DPSCs. This FA-modified PCL nanofiber scaffold shows promising potential for future bone, dental, and orthopedic regenerative applications.

Indexed as

Alkaline PhosphataseApatitesCalcification, PhysiologicCell AdhesionCell AggregationCell Culture TechniquesCell ProliferationCellular MicroenvironmentCollagen Type ICollagen Type I, alpha 1 ChainCore Binding Factor Alpha 1 SubunitCrystallizationDental PulpDentin SialophosphoproteinExtracellular Matrix ProteinsHumansAlkaline PhosphataseApatitesCollagen Type ICollagen Type I, alpha 1 ChainCore Binding Factor Alpha 1 SubunitDentin SialophosphoproteinDMP1 protein, humanExtracellular Matrix ProteinsfluorapatiteOsteocalcinOsteopontinPhosphoproteinspolycaprolactonePolyestersRUNX2 protein, humanSialoglycoproteinsSPP1 protein, humanbiomaterial(s)cell differentiationnanofibersodontogenesisosteogenesistissue engineering

Identifiers

PMID25139361
PMCPMC4462802
OpenAlexW2136144194

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.