ArticlePlant physiology2014
A secreted protein with plant-specific cysteine-rich motif functions as a mannose-binding lectin that exhibits antifungal activity.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 123 citations in OpenAlex.
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- Genetic Diversity, Predictive Protein Structures, and Interaction Networks of Cysteine-Rich Receptor-Like Kinases inComputational and structural biotechnology journal · 2026Article
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- A retrotransposon insertion upstream of Arabidopsis thaliana CRK8 receptor-like kinase modulates a trade-off between pathogen defense and salt tolerance.The Plant journal : for cell and molecular biology · 2025Article
- Single-cell transcriptome atlas unveils transcriptional regulation networks of banana root tips in response toHorticulture research · 2025Article
- Cysteine-rich receptor-like secreted protein 1 promotes intercellular infection and enhances nodulation inHorticulture research · 2025Article
- Proteomic snapshot of pattern triggered immunity in the Arabidopsis leaf apoplast.The Plant journal : for cell and molecular biology · 2025Article
- More questions than answers: insights into potential cysteine-rich receptor-like kinases redox signalling in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Review
- Septoria tritici blotch resistance gene Stb15 encodes a lectin receptor-like kinase.Nature plants · 2025Article
- Tandem kinase proteins across the plant kingdom.Nature genetics · 2025Article
- Transcriptomic Profile of Tef (Plants (Basel, Switzerland) · 2024Article
- The CRK14 gene encoding a cysteine-rich receptor-like kinase is implicated in the regulation of global proliferative arrest in Arabidopsis thaliana.Genes to cells : devoted to molecular & cellular mechanisms · 2024Article
- Overexpression of Ginkbilobin-2 homologous domain gene improves tolerance to Phytophthora cinnamomi in somatic embryos of Quercus suber.Scientific reports · 2024Article
- Evolutionary assembly of the plant terrestrialization toolkit from protein domains.Proceedings. Biological sciences · 2024Article
- A haplotype-resolved chromosome-level assembly and annotation of European hazelnut (C. avellana cv. Jefferson) provides insight into mechanisms of eastern filbert blight resistance.G3 (Bethesda, Md.) · 2024Article
- Review
- Emerging Roles of Receptor-like Protein Kinases in Plant Response to Abiotic Stresses.International journal of molecular sciences · 2023Review
- Article
Corrections and comments
- Erratum issuedCorrections.2015
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants have a variety of mechanisms for defending against plant pathogens and tolerating environmental stresses such as drought and high salinity. Ginkbilobin2 (Gnk2) is a seed storage protein in gymnosperm that possesses antifungal activity and a plant-specific cysteine-rich motif (domain of unknown function26 [DUF26]). The Gnk2-homologous sequence is also observed in an extracellular region of cysteine-rich repeat receptor-like kinases that function in response to biotic and abiotic stresses. Here, we report the lectin-like molecular function of Gnk2 and the structural basis of its monosaccharide recognition. Nuclear magnetic resonance experiments showed that mannan was the only yeast (Saccharomyces cerevisiae) cell wall polysaccharide that interacted with Gnk2. Gnk2 also interacted with mannose, a building block of mannan, with a specificity that was similar to those of mannose-binding legume lectins, by strictly recognizing the configuration of the hydroxy group at the C4 position of the monosaccharide. The crystal structure of Gnk2 in complex with mannose revealed that three residues (asparagine-11, arginine-93, and glutamate-104) recognized mannose by hydrogen bonds, which defined the carbohydrate-binding specificity. These interactions were directly related to the ability of Gnk2 to inhibit the growth of fungi, including the plant pathogenic Fusarium spp., which were disrupted by mutation of arginine-93 or the presence of yeast mannan in the assay system. In addition, Gnk2 did not inhibit the growth of a yeast mutant strain lacking the α1,2-linked mannose moiety. These results provide insights into the molecular basis of the DUF26 protein family.
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