ArticleAging cell2014
Muscle ectopic fat deposition contributes to anabolic resistance in obese sarcopenic old rats through eIF2α activation.
Article in Aging cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers, 4 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
90 citing papers in PubMed, 4 syntheses or guidelines pooled it, 189 citations in OpenAlex.
- Associations between the triglyceride‒glucose index and the odds of low muscle mass and sarcopenia: a systematic review and meta-analysis.BMC endocrine disorders · 2026Pooled it
- Pooled it
- Effect of resistance training on body composition and physical function in older females with sarcopenic obesity-a systematic review and meta-analysis of randomized controlled trials.Frontiers in aging neuroscience · 2025Pooled it
- Effect of flavonoids on skeletal muscle mass, strength and physical performance in middle-aged and older adults with or without Sarcopenia: A meta-analysis of randomized controlled trials.Frontiers in nutrition · 2022Pooled it
- Omega-3 Supplementation Improves Isometric Strength But Not Muscle Anabolic and Catabolic Signaling in Response to Resistance Exercise in Healthy Older Adults.The journals of gerontology. Series A, Biological sciences and medical sciences · 2021Trial
- The association between composite dietary antioxidant index and sarcopenic obesity.Nutrition & metabolism · 2025Article
- Age-Related Anabolic Resistance: Nutritional and Exercise Strategies, and Potential Relevance to Life-Long Exercisers.Nutrients · 2025Review
- Effects of dietary camelina oil intake on skeletal muscle metabolism and sarcopenia in older mice.Journal of physiology and biochemistry · 2025Article
- Preclinical research in obesity-associated metabolic diseases using in vitro, multicellular, and non-mammalian models.Journal of physiology and biochemistry · 2025Review
- Muscle memory of exercise optimizes mitochondrial metabolism to support skeletal muscle growth.American journal of physiology. Cell physiology · 2025Article
- Low skeletal muscle mass independently predicts the progression of insulin resistance in non-obese older adults: a six-year cohort study.Diabetology international · 2025Article
- Overexpression of the human heat shock protein B1 alters obesity-related metabolic changes in a sex-dependent manner in a mouse model of metabolic syndrome.Biology of sex differences · 2025Article
- Voluntary Exercise-Induced Skeletal Muscle Responses in Young and Aged Mice on a High-Fat Diet.Cureus · 2025Article
- Effects of Sleeve Gastrectomy and Treadmill Exercise on Skeletal Muscle and Ectopic Fat in High-Fat Diet-Induced Obese Rats.International journal of molecular sciences · 2025Article
- Sarcopenic obesity: a review.Archives of endocrinology and metabolism · 2025Review
- Body composition alterations in patients with lung cancer.American journal of physiology. Cell physiology · 2025Review
- The impact of elevated dietary inflammatory potential on sarcopenic obesity: evidence from two observational studies.Frontiers in nutrition · 2025Article
- Probiotics and muscle health: the impact ofFrontiers in microbiology · 2025Review
- Geriatric nutritional risk index and body composition dictate the prognosis of elderly patients with intrahepatic cholangiocarcinoma.Frontiers in nutrition · 2025Article
- Age-Related Changes in Insulin Resistance and Muscle Mass: Clinical Implications in Obese Older Adults.Medicina (Kaunas, Lithuania) · 2024Review
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
14 authors at 6 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obesity and aging are characterized by decreased insulin sensitivity (IS) and muscle protein synthesis. Intramuscular ceramide accumulation has been implicated in insulin resistance during obesity. We aimed to measure IS, muscle ceramide level, protein synthesis, and activation of intracellular signaling pathways involved in translation initiation in male Wistar young (YR, 6-month) and old (OR, 25-month) rats receiving a low- (LFD) or a high-fat diet (HFD) for 10 weeks. A corresponding cellular approach using C2C12 myotubes treated with palmitate to induce intracellular ceramide deposition was taken. A decreased ability of adipose tissue to store lipids together with a reduced adipocyte diameter and a development of fibrosis were observed in OR after the HFD. Consequently, OR fed the HFD were insulin resistant, showed a strong increase in intramuscular ceramide level and a decrease in muscle protein synthesis associated with increased eIF2α phosphorylation. The accumulation of intramuscular lipids placed a lipid burden on mitochondria and created a disconnect between metabolic and regulating pathways in skeletal muscles of OR. In C2C12 cells, palmitate-induced ceramide accumulation was associated with a decreased protein synthesis together with upregulated eIF2α phosphorylation. In conclusion, a reduced ability to expand adipose tissues was found in OR, reflecting a lower lipid buffering capacity. Muscle mitochondrial activity was affected in OR conferring a reduced ability to oxidize fatty acids entering the muscle cell. Hence, OR were more prone to ectopic muscle lipid accumulation than YR, leading to decreased muscle protein anabolism. This metabolic change is a potential therapeutic target to counter sarcopenic obesity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.