ArticleMolecular microbiology2014
MG428 is a novel positive regulator of recombination that triggers mgpB and mgpC gene variation in Mycoplasma genitalium.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 21 citations in OpenAlex.
- Optimization and Clinical Evaluation of an Automated Commercial Analyte-Specific Reagent Assay forJournal of clinical microbiology · 2023Article
- Unveiling the stealthy tactics: mycoplasma's immune evasion strategies.Frontiers in cellular and infection microbiology · 2023Review
- Pathogenicity and virulence ofVirulence · 2022Review
- TheAntibiotics (Basel, Switzerland) · 2021Article
- The mechanisms underlying antigenic variation and maintenance of genomic integrity in Mycoplasma pneumoniae and Mycoplasma genitalium.Archives of microbiology · 2021Review
- Protein quality control and regulated proteolysis in the genome-reduced organism Mycoplasma pneumoniae.Molecular systems biology · 2020Article
- Article
- Determination of the Gene Regulatory Network of a Genome-Reduced Bacterium Highlights Alternative Regulation Independent of Transcription Factors.Cell systems · 2019Article
- Subversion of the Immune Response by Human Pathogenic Mycoplasmas.Frontiers in microbiology · 2019Review
- Activation of σ20-dependent recombination and horizontal gene transfer in Mycoplasma genitalium.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- Mycoplasma genitalium Nonadherent Phase Variants Arise by Multiple Mechanisms and Escape Antibody-Dependent Growth Inhibition.Infection and immunity · 2018Article
- The Unique Microbiology and Molecular Pathogenesis of Mycoplasma genitalium.The Journal of infectious diseases · 2017Review
- Potential Molecular Targets for Narrow-Spectrum Agents to Combat Mycoplasma pneumoniae Infection and Disease.Frontiers in microbiology · 2016Review
- A novel sigma factor reveals a unique regulon controlling cell-specific recombination in Mycoplasma genitalium.Nucleic acids research · 2015Article
- Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The human pathogen Mycoplasma genitalium employs homologous recombination to generate antigenic diversity in the immunodominant MgpB and MgpC proteins. Only recently, some of the molecular factors involved in this process have been characterized, but nothing is known about its regulation. Here, we show that M. genitalium expresses N-terminally truncated RecA isoforms via alternative translation initiation, but only the full-length protein is essential for gene variation. We also demonstrate that overexpression of MG428 positively regulates the expression of recombination genes, including recA, ruvA, ruvB and ORF2, a gene of unknown function co-transcribed with ruvAB. The co-ordinated induction of these genes correlated with an increase of mgpBC gene variation. In contrast, cells lacking MG428 were unable to generate variants despite expressing normal levels of RecA. Similarly, deletion analyses of the recA upstream region defined sequences required for gene variation without abolishing RecA expression. The requirement of these sequences is consistent with the presence of promoter elements associated with MG428-dependent recA induction. Sequences upstream of recA also influence the relative abundance of RecA isoforms, possibly through translational regulation. Overall, these results suggest that MG428 is a positive regulator of recombination and that precise control of recA expression is required to initiate mgpBC variation.
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