ArticlePhysiological reports2014
The exocyst and regulatory GTPases in urinary exosomes.
Article in Physiological reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Modeling Complex Developmental Disease: The Case of Polycystic Kidney Disease.Journal of developmental biology · 2026Review
- The exocyst in ciliogenesis.Renal failure · 2025Review
- Review
- Structure and interactions of the endogenous human Commander complex.Nature structural & molecular biology · 2024Article
- Emerging principles of primary cilia dynamics in controlling tissue organization and function.The EMBO journal · 2023Review
- Tracking N- and C-termini of C. elegans polycystin-1 reveals their distinct targeting requirements and functions in cilia and extracellular vesicles.PLoS genetics · 2022Article
- Reduced endosomal microautophagy activity in aging associates with enhanced exocyst-mediated protein secretion.Aging cell · 2022Article
- Shedding of ciliary vesicles at a glance.Journal of cell science · 2022Article
- Exocyst controls exosome biogenesis via Rab11a.Molecular therapy. Nucleic acids · 2022Article
- EV duty vehicles: Features and functions of ciliary extracellular vesicles.Frontiers in genetics · 2022Review
- Extracellular Vesicles: A Novel Tool Facilitating Personalized Medicine and Pharmacogenomics in Oncology.Frontiers in pharmacology · 2021Review
- Insights Into the Molecular Mechanisms of Polycystic Kidney Diseases.Frontiers in physiology · 2021Review
- Primary cilia and the exocyst are linked to urinary extracellular vesicle production and content.The Journal of biological chemistry · 2019Article
- The role of the exocyst in renal ciliogenesis, cystogenesis, tubulogenesis, and development.Kidney research and clinical practice · 2019Review
- Turnip Mosaic Virus Components Are Released into the Extracellular Space by Vesicles in Infected Leaves.Plant physiology · 2019Article
- Cell-cell communication via ciliary extracellular vesicles: clues from model systems.Essays in biochemistry · 2018Review
- Ciliary Extracellular Vesicles: Txt Msg Organelles.Cellular and molecular neurobiology · 2016Review
- Targeting vascular and leukocyte communication in angiogenesis, inflammation and fibrosis.Nature reviews. Drug discovery · 2016Review
- The Exocyst Complex in Health and Disease.Frontiers in cell and developmental biology · 2016Review
- Primary cilia and autophagic dysfunction in Huntington's disease.Cell death and differentiation · 2015Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Cilia, organelles that function as cellular antennae, are central to the pathogenesis of "ciliopathies", including various forms of polycystic kidney disease (PKD). To date, however, the molecular mechanisms controlling ciliogenesis and ciliary function remain incompletely understood. A recently proposed model of cell-cell communication, called "urocrine signaling", hypothesizes that a subset of membrane bound vesicles that are secreted into the urinary stream (termed exosome-like vesicles, or ELVs), carry cilia-specific proteins as cargo, interact with primary cilia, and affect downstream cellular functions. This study was undertaken to determine the role of the exocyst, a highly conserved eight-protein trafficking complex, in the secretion and/or retrieval of ELVs. We used Madin-Darby canine kidney (MDCK) cells expressing either Sec10-myc (a central component of the exocyst complex) or Smoothened-YFP (a ciliary protein found in ELVs) in experiments utilizing electron gold microscopy and live fluorescent microscopy, respectively. Additionally, human urinary exosomes were isolated via ultracentrifugation and subjected to mass-spectrometry-based proteomics analysis to determine the composition of ELVs. We found, as determined by EM, that the exocyst localizes to primary cilia, and is present in vesicles attached to the cilium. Furthermore, the entire exocyst complex, as well as most of its known regulatory GTPases, are present in human urinary ELVs. Finally, in living MDCK cells, ELVs appear to interact with primary cilia using spinning disc confocal microscopy. These data suggest that the exocyst complex, in addition to its role in ciliogenesis, is centrally involved in the secretion and/or retrieval of urinary ELVs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.