ArticleBrain pathology (Zurich, Switzerland)2015
Injury Response of Resected Human Brain Tissue In Vitro.
Article in Brain pathology (Zurich, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Live-cell physiology in human brain tissue culture-the potential, the challenges, and the lessons learned.Frontiers in cellular neuroscience · 2026Review
- From mice to clinical relevance: humanizing neuroscience with human-based model systems.Frontiers in cellular neuroscience · 2026Review
- Divergent actions of physiological and pathological amyloid-β on synapses in live human brain slice cultures.Nature communications · 2025Article
- Astrocytes in Primary Familial Brain Calcification (PFBC): Emphasis on the Importance of Induced Pluripotent Stem Cell-Derived Human Astrocyte Models.Advances in experimental medicine and biology · 2025Review
- Modeling the Human Brain WithFrontiers in neuroscience · 2022Article
- The Potential of Induced Pluripotent Stem Cells to Treat and Model Alzheimer's Disease.Stem cells international · 2021Review
- Grafted human pluripotent stem cell-derived cortical neurons integrate into adult human cortical neural circuitry.Stem cells translational medicine · 2020Article
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
- Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds.Neuroscience bulletin · 2019Review
- Doublecortin-expressing cell types in temporal lobe epilepsy.Acta neuropathologica communications · 2018Article
- Alterations in the steroid biosynthetic pathways in the human prefrontal cortex in mood disorders: A post-mortem study.Brain pathology (Zurich, Switzerland) · 2018Article
- Article
- Altered Loyalties of Neuronal Markers in Cultured Slices of Resected Human Brain Tissue.Brain pathology (Zurich, Switzerland) · 2016Article
- Hippocampus and epilepsy: Findings from human tissues.Revue neurologique · 2015Review
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brain injury affects a significant number of people each year. Organotypic cultures from resected normal neocortical tissue provide unique opportunities to study the cellular and neuropathological consequences of severe injury of adult human brain tissue in vitro. The in vitro injuries caused by resection (interruption of the circulation) and aggravated by the preparation of slices (severed neuronal and glial processes and blood vessels) reflect the reaction of human brain tissue to severe injury. We investigated this process using immunocytochemical markers, reverse transcriptase quantitative polymerase chain reaction and Western blot analysis. Essential features were rapid shrinkage of neurons, loss of neuronal marker expression and proliferation of reactive cells that expressed Nestin and Vimentin. Also, microglia generally responded strongly, whereas the response of glial fibrillary acidic protein-positive astrocytes appeared to be more variable. Importantly, some reactive cells also expressed both microglia and astrocytic markers, thus confounding their origin. Comparison with post-mortem human brain tissue obtained at rapid autopsies suggested that the reactive process is not a consequence of epilepsy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.