ArticleAnalytical chemistry2014
Droplet electrospray ionization mass spectrometry for high throughput screening for enzyme inhibitors.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 86 citations in OpenAlex.
- High-Throughput Screening of Isomeric Reaction Products by Droplet Microfluidics Coupled to Cyclic Ion Mobility-Mass Spectrometry.Analytical chemistry · 2026Article
- Utilizing Venturi effect for automated high-throughput droplet-MS from well plates.The Analyst · 2026Article
- Droplet-Based Microfluidics Methods for Detecting Enzyme Inhibitors.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Bone-on-a-Chip Systems for Hematological Cancers.Biosensors · 2025Review
- 1-Octanol-assisted ultra-small volume droplet microfluidics with nanoelectrospray ionization mass spectrometry.Analytica chimica acta · 2024Article
- Coupling of droplet-on-demand microfluidcs with ESI/MS to study single-cell catalysis.RSC advances · 2024Article
- Microdialysis coupled with droplet microfluidics and mass spectrometry for determination of neurotransmittersThe Analyst · 2024Article
- Uncovering Distinct Peptide Charging Behaviors in Electrospray Ionization Mass Spectrometry Using a Large-Scale Dataset.Journal of the American Society for Mass Spectrometry · 2024Article
- High-Throughput Liquid Chromatographic Analysis Using a Segmented Flow Injector with a 1 s Cycle Time.Analytical chemistry · 2023Article
- Programmable Control of Nanoliter Droplet Arrays using Membrane Displacement Traps.Advanced materials technologies · 2023Article
- Ultrahigh-Throughput Enzyme Engineering and Discovery inChemical reviews · 2023Review
- A high-throughput approach reveals distinct peptide charging behaviors in electrospray ionization mass spectrometry.bioRxiv : the preprint server for biology · 2023Article
- Discovery of Novel Small-Molecule Scaffolds for the Inhibition and Activation of WIP1 Phosphatase from a RapidFire Mass Spectrometry High-Throughput Screen.ACS pharmacology & translational science · 2022Article
- Analysis of double-emulsion droplets with ESI mass spectrometry for monitoring lipase-catalyzed ester hydrolysis at nanoliter scale.Analytical and bioanalytical chemistry · 2022Article
- Droplet-assisted electrospray phase separation using an integrated silicon microfluidic platform.Lab on a chip · 2021Article
- Droplet Microfluidics and Directed Evolution of Enzymes: An Intertwined Journey.Angewandte Chemie (International ed. in English) · 2021Review
- Multi-Organs-on-Chips for Testing Small-Molecule Drugs: Challenges and Perspectives.Pharmaceutics · 2021Review
- Faster, better, and cheaper: harnessing microfluidics and mass spectrometry for biotechnology.RSC chemical biology · 2021Review
- Comparison of Design Approaches for Low-Cost Sampling Mechanisms in Open-Source Chemical Instrumentation.HardwareX · 2021Article
- Rapid Optimization of Photoredox Reactions for Continuous-Flow Systems Using Microscale Batch Technology.ACS central science · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
High throughput screening (HTS) is important for identifying molecules with desired properties. Mass spectrometry (MS) is potentially powerful for label-free HTS due to its high sensitivity, speed, and resolution. Segmented flow, where samples are manipulated as droplets separated by an immiscible fluid, is an intriguing format for high throughput MS because it can be used to reliably and precisely manipulate nanoliter volumes and can be directly coupled to electrospray ionization (ESI) MS for rapid analysis. In this study, we describe a "MS Plate Reader" that couples standard multiwell plate HTS workflow to droplet ESI-MS. The MS plate reader can reformat 3072 samples from eight 384-well plates into nanoliter droplets segmented by an immiscible oil at 4.5 samples/s and sequentially analyze them by MS at 2 samples/s. Using the system, a label-free screen for cathepsin B modulators against 1280 chemicals was completed in 45 min with a high Z-factor (>0.72) and no false positives (24 of 24 hits confirmed). The assay revealed 11 structures not previously linked to cathepsin inhibition. For even larger scale screening, reformatting and analysis could be conducted simultaneously, which would enable more than 145,000 samples to be analyzed in 1 day.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.