ArticlePLoS biology2014
The cytoplasmic capping complex assembles on adapter protein nck1 bound to the proline-rich C-terminus of Mammalian capping enzyme.
Article in PLoS biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- RNMT-dependent RNA cap methylation in health and disease.The Biochemical journal · 2025Review
- RNA modifications and their role in gene expression.Frontiers in molecular biosciences · 2025Review
- METTL3 alters capping enzyme expression and its activity on ribosomal proteins.Scientific reports · 2024Article
- Widespread 3'UTR capped RNAs derive from G-rich regions in proximity to AGO2 binding sites.BMC biology · 2024Article
- Trypanosome mRNA recapping is triggered by hypermethylation originating from cap 4.Nucleic acids research · 2024Article
- METTL3 alters capping enzyme expression and its activity on ribosomal proteins.bioRxiv : the preprint server for biology · 2023Article
- Long noncoding RNAs are substrates for cytoplasmic capping enzyme.FEBS letters · 2023Article
- Nck adaptors at a glance.Journal of cell science · 2021Article
- A novel RNA pol II CTD interaction site on the mRNA capping enzyme is essential for its allosteric activation.Nucleic acids research · 2021Article
- NCK1-AS1 promotes the progression of lung squamous cell carcinoma through transcriptionally upregulating NCK1 via interacting with MYC.Cancer biology & therapy · 2021Article
- Cytoplasmic mRNA recapping has limited impact on proteome complexity.Open biology · 2020Article
- Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.Nucleic acids research · 2020Article
- A Benzophenone-Based Photocaging Strategy for the N7 Position of Guanosine.Angewandte Chemie (International ed. in English) · 2020Article
- mRNA 5' ends targeted by cytoplasmic recapping cluster at CAGE tags and select transcripts are alternatively spliced.FEBS letters · 2019Article
- A recap of RNA recapping.Wiley interdisciplinary reviews. RNA · 2019Review
- RNA-binding proteins and heat-shock protein 90 are constituents of the cytoplasmic capping enzyme interactome.The Journal of biological chemistry · 2018Article
- Article
- Peptide-based sequestration of the adaptor protein Nck1 in pancreatic β cells enhances insulin biogenesis and protects against diabetogenic stresses.The Journal of biological chemistry · 2018Article
- RNA guanine-7 methyltransferase catalyzes the methylation of cytoplasmically recapped RNAs.Nucleic acids research · 2017Article
- African Swine Fever Virus NP868R Capping Enzyme Promotes Reovirus Rescue during Reverse Genetics by Promoting Reovirus Protein Expression, Virion Assembly, and RNA Incorporation into Infectious Virions.Journal of virology · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cytoplasmic capping is catalyzed by a complex that contains capping enzyme (CE) and a kinase that converts RNA with a 5'-monophosphate end to a 5' diphosphate for subsequent addition of guanylic acid (GMP). We identify the proline-rich C-terminus as a new domain of CE that is required for its participation in cytoplasmic capping, and show the cytoplasmic capping complex assembles on Nck1, an adapter protein with functions in translation and tyrosine kinase signaling. Binding is specific to Nck1 and is independent of RNA. We show by sedimentation and gel filtration that Nck1 and CE are together in a larger complex, that the complex can assemble in vitro on recombinant Nck1, and Nck1 knockdown disrupts the integrity of the complex. CE and the 5' kinase are juxtaposed by binding to the adjacent domains of Nck1, and cap homeostasis is inhibited by Nck1 with inactivating mutations in each of these domains. These results identify a new domain of CE that is specific to its function in cytoplasmic capping, and a new role for Nck1 in regulating gene expression through its role as the scaffold for assembly of the cytoplasmic capping complex.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.