Evidence map›Paper›PMID 25136492›Full record

ArticleBiomedical optics express2014

Calcium imaging at kHz frame rates resolves millisecond timing in neuronal circuits and varicosities.

Michiel A Martens, Werend Boesmans, Pieter Vanden Berghe

Abstract read
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In one paragraph

Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Optical Tools to Investigate Cellular Activity in the Intestinal Wall.Journal of neurogastroenterology and motility · 2015
    Article
  6. Changes in Nicotinic Neurotransmission during Enteric Nervous System Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Michiel A MartensLaboratory for Enteric Neuroscience (LENS),TARGID, University of Leuven, O&N 1 Herestraat 49-box 701, Leuven 3000, Belgium.
Werend BoesmansLaboratory for Enteric Neuroscience (LENS),TARGID, University of Leuven, O&N 1 Herestraat 49-box 701, Leuven 3000, Belgium.
Pieter Vanden BergheLaboratory for Enteric Neuroscience (LENS),TARGID, University of Leuven, O&N 1 Herestraat 49-box 701, Leuven 3000, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have configured a widefield fast imaging system that allows imaging at 1000 frames per second (512x512 pixels). The system was extended with custom processing tools including a time correlation method to facilitate the analysis of static subcellular compartments (e.g. neuronal varicosities) with enhanced contrast, as well as a dynamic intensity processing (DIP) algorithm that aids in data size reduction and fast visualization and interpretation of timing and directionality in neuronal circuits. This system, together with our custom developed processing tools enables efficient detection of fast physiological events, such as action potential dependent calcium steps. We show, using a specific blocker of nerve communication, that with this setup it is possible to discriminate between a pre and post synaptic event in an all optical way.

Indexed as

(100.0118) Imaging ultrafast phenomena(100.2000) Digital image processing(170.6920) Time-resolved imaging(330.6790) Temporal discrimination

Identifiers

PMID25136492
PMCPMC4132995

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.