Evidence map›Paper›PMID 25136483›Full record

ArticleBiomedical optics express2014

Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device.

Li-Chung Cheng, Chi-Hsiang Lien, Yong Da Sie, Yvonne Yuling Hu, Chun-Yu Lin, Fan-Ching Chien, Chris Xu, Chen Yuan Dong, Shean-Jen Chen

Abstract read
In one paragraph

Article in Biomedical optics express, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Li-Chung ChengDepartment of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
Chi-Hsiang LienDepartment of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Yong Da SieDepartment of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Yvonne Yuling HuDepartment of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Chun-Yu LinDepartment of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
Fan-Ching ChienDepartment of Optics and Photonics, National Central University, Jhongli 320, Taiwan.
Chris XuSchool of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA.
Chen Yuan DongDepartment of Physics, National Taiwan University, Taipei 106, Taiwan ; cydong@phys.ntu.edu.tw.
Shean-Jen ChenDepartment of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan ; Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan ; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, the light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously and accurately implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order NSIM and projected structured light, respectively. The experimental results demonstrate that the lateral and axial resolutions are enhanced from 397 nm to 168 nm (2.4-fold) and from 2.33 μm to 1.22 μm (1.9-fold), respectively, in full width at the half maximum. Furthermore, a three-dimensionally rendered image of a cytoskeleton cell featuring ~25 nm microtubules is improved, with other microtubules at a distance near the lateral resolution of 168 nm also able to be distinguished.

Indexed as

(170.3880) Medical and biological imaging(180.4315) Nonlinear microscopy(190.4180) Multiphoton processes

Identifiers

PMID25136483
PMCPMC4132986

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.