ArticleProceedings of the National Academy of Sciences of the United States of America2014
Stimulation of growth by proteorhodopsin phototrophy involves regulation of central metabolic pathways in marine planktonic bacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 118 citations in OpenAlex.
- Major contribution of anaplerosis to inorganic carbon fixation in the dark ocean.Nature geoscience · 2026Article
- Priority effects inhibit the repeated evolution of phototrophy.Npj complexity · 2026Article
- Two newFEMS microbes · 2026Article
- Ecogenomics and functional biogeography of the Roseobacter group in the global oceans based on 653 MAGs and SAGs.Microbiome · 2025Article
- Carotenoids bind rhodopsins and act as photocycle-accelerating pigments in marine Bacteroidota.Nature microbiology · 2025Article
- Effect of Light Regime onJournal of microbiology and biotechnology · 2024Article
- Long-Time Scale Simulations Reveal Key Dynamics That Drive the Onset of the N State in the Proteorhodopsin Photocycle.The journal of physical chemistry. B · 2024Article
- Rhodopsin-based light-harvesting system for sustainable synthetic biology.Microbial biotechnology · 2024Article
- Niche differentiation within bacterial key-taxa in stratified surface waters of the Southern Pacific Gyre.The ISME journal · 2024Article
- Viruses under the Antarctic Ice Shelf are active and potentially involved in global nutrient cycles.Nature communications · 2023Article
- Engineering artificial photosynthesis based on rhodopsin for CONature communications · 2023Article
- A comparative study reveals the relative importance of prokaryotic and eukaryotic proton pump rhodopsins in a subtropical marginal sea.ISME communications · 2023Article
- Rhodopsin driven microbial COEnvironmental microbiology · 2023Article
- Emerging Research Topics in the Vibrionaceae and the Squid-Microorganisms · 2022Review
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- Functional responses of key marine bacteria to environmental change - toward genetic counselling for coastal waters.Frontiers in microbiology · 2022Article
- Revealing COBiodesign research · 2022Article
- Highly integrated adaptive mechanisms in Spiribacter halalkaliphilus, a bacterium abundant in Chinese soda-saline lakes.Environmental microbiology · 2021Article
- Mixotrophic bacteria for environmental detoxification of contaminated waste and wastewater.Applied microbiology and biotechnology · 2021Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteorhodopsin (PR) is present in half of surface ocean bacterioplankton, where its light-driven proton pumping provides energy to cells. Indeed, PR promotes growth or survival in different bacteria. However, the metabolic pathways mediating the light responses remain unknown. We analyzed growth of the PR-containing Dokdonia sp. MED134 (where light-stimulated growth had been found) in seawater with low concentrations of mixed [yeast extract and peptone (YEP)] or single (alanine, Ala) carbon compounds as models for rich and poor environments. We discovered changes in gene expression revealing a tightly regulated shift in central metabolic pathways between light and dark conditions. Bacteria showed relatively stronger light responses in Ala compared with YEP. Notably, carbon acquisition pathways shifted toward anaplerotic CO2 fixation in the light, contributing 31 ± 8% and 24 ± 6% of the carbon incorporated into biomass in Ala and YEP, respectively. Thus, MED134 was a facultative double mixotroph, i.e., photo- and chemotrophic for its energy source and using both bicarbonate and organic matter as carbon sources. Unexpectedly, relative expression of the glyoxylate shunt genes (isocitrate lyase and malate synthase) was >300-fold higher in the light--but only in Ala--contributing a more efficient use of carbon from organic compounds. We explored these findings in metagenomes and metatranscriptomes and observed similar prevalence of the glyoxylate shunt compared with PR genes and highest expression of the isocitrate lyase gene coinciding with highest solar irradiance. Thus, regulatory interactions between dissolved organic carbon quality and central metabolic pathways critically determine the fitness of surface ocean bacteria engaging in PR phototrophy.
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