ArticleMolecular biology and evolution2014
Integrating evolutionary and functional tests of adaptive hypotheses: a case study of altitudinal differentiation in hemoglobin function in an Andean Sparrow, Zonotrichia capensis.
Article in Molecular biology and evolution, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Article
- Convergent High OAnimals : an open access journal from MDPI · 2024Article
- Genomic Variation, Population History, and Long-Term Genetic Adaptation to High Altitudes in Tibetan Partridge (Perdix hodgsoniae).Molecular biology and evolution · 2023Article
- Comparison between short-term stress and long-term adaptive responses reveal common paths to molecular adaptation.iScience · 2022Article
- Genetic variation in haemoglobin is associated with evolved changes in breathing in high-altitude deer mice.The Journal of experimental biology · 2022Article
- High-Altitude Adaptation: Mechanistic Insights from Integrated Genomics and Physiology.Molecular biology and evolution · 2021Review
- The adaptive benefit of evolved increases in hemoglobin-OBMC biology · 2021Article
- The evolution of ancestral and species-specific adaptations in snowfinches at the Qinghai-Tibet Plateau.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Evolved increases in hemoglobin-oxygen affinity and the Bohr effect coincided with the aquatic specialization of penguins.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Physiological Genomics of Adaptation to High-Altitude Hypoxia.Annual review of animal biosciences · 2021Review
- Differential Expression of Metabolism-Related Genes in Plateau Pika (Frontiers in genetics · 2021Article
- Effect of NH2-terminal acetylation on the oxygenation properties of vertebrate haemoglobin.The Biochemical journal · 2020Article
- Synthesis of Recombinant Human Hemoglobin With NHCurrent protocols in protein science · 2020Article
- Biochemical pedomorphosis and genetic assimilation in the hypoxia adaptation of Tibetan antelope.Science advances · 2020Article
- Rapid phenotypic evolution with shallow genomic differentiation during early stages of high elevation adaptation in Eurasian Tree Sparrows.National science review · 2020Article
- The role of mutation bias in adaptive molecular evolution: insights from convergent changes in protein function.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Evolution of physiological performance capacities and environmental adaptation: insights from high-elevation deer mice (Journal of mammalogy · 2019Article
- Elevational divergence in the great tit complex revealed by major hemoglobin genes.Current zoology · 2018Article
- Neutral and functionally important genes shed light on phylogeography and the history of high-altitude colonization in a widespread New World duck.Ecology and evolution · 2018Article
- Molecular basis of hemoglobin adaptation in the high-flying bar-headed goose.PLoS genetics · 2018Article
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11 authors.
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Abstract
In air-breathing vertebrates, the physiologically optimal blood-O2 affinity is jointly determined by the prevailing partial pressure of atmospheric O2, the efficacy of pulmonary O2 transfer, and internal metabolic demands. Consequently, genetic variation in the oxygenation properties of hemoglobin (Hb) may be subject to spatially varying selection in species with broad elevational distributions. Here we report the results of a combined functional and evolutionary analysis of Hb polymorphism in the rufous-collared sparrow (Zonotrichia capensis), a species that is continuously distributed across a steep elevational gradient on the Pacific slope of the Peruvian Andes. We integrated a population genomic analysis that included all postnatally expressed Hb genes with functional studies of naturally occurring Hb variants, as well as recombinant Hb (rHb) mutants that were engineered through site-directed mutagenesis. We identified three clinally varying amino acid polymorphisms: Two in the α(A)-globin gene, which encodes the α-chain subunits of the major HbA isoform, and one in the α(D)-globin gene, which encodes the α-chain subunits of the minor HbD isoform. We then constructed and experimentally tested single- and double-mutant rHbs representing each of the alternative α(A)-globin genotypes that predominate at different elevations. Although the locus-specific patterns of altitudinal differentiation suggested a history of spatially varying selection acting on Hb polymorphism, the experimental tests demonstrated that the observed amino acid mutations have no discernible effect on respiratory properties of the HbA or HbD isoforms. These results highlight the importance of experimentally validating the hypothesized effects of genetic changes in protein function to avoid the pitfalls of adaptive storytelling.
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