ArticleBMC genomics2014
Species and gene divergence in Littorina snails detected by array comparative genomic hybridization.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Chromosomal inversion polymorphisms are widespread across the species ranges of rough periwinkles (Littorina saxatilis and L. arcana).Molecular ecology · 2024Article
- Gut Bacteriomes and Ecological Niche Divergence: An Example of Two Cryptic Gastropod Species.Biology · 2023Article
- First insights into the gut microbiomes and the diet of theEvolutionary applications · 2023Article
- Linking ecology, morphology, and metabolism: Niche differentiation in sympatric populations of closely related species of the genusEcology and evolution · 2021Article
- Sticky problems: extraction of nucleic acids from molluscs.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- Correspondence of aCGH and long-read genome assembly for detection of copy number differences: A proof-of-concept with cichlid genomes.PloS one · 2021Article
- Divergence together with microbes: A comparative study of the associated microbiomes in the closely related Littorina species.PloS one · 2021Article
- Article
- The Genomic Substrate for Adaptive Radiation: Copy Number Variation across 12 Tribes of African Cichlid Species.Genome biology and evolution · 2019Article
- Population genomics of parallel evolution in gene expression and gene sequence during ecological adaptation.Scientific reports · 2018Article
- Article
- Evaluation of Apis mellifera syriaca Levant region honeybee conservation using comparative genome hybridization.Genetica · 2016Article
- Divergence within and among Seaweed Siblings (Fucus vesiculosus and F. radicans) in the Baltic Sea.PloS one · 2016Article
- What can be learnt from a snail?Evolutionary applications · 2016Review
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8 authors.
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Abstract
backgroundArray comparative genomic hybridization (aCGH) is commonly used to screen different types of genetic variation in humans and model species. Here, we performed aCGH using an oligonucleotide gene-expression array for a non-model species, the intertidal snail Littorina saxatilis. First, we tested what types of genetic variation can be detected by this method using direct re-sequencing and comparison to the Littorina genome draft. Secondly, we performed a genome-wide comparison of four closely related Littorina species: L. fabalis, L. compressa, L. arcana and L. saxatilis and of populations of L. saxatilis found in Spain, Britain and Sweden. Finally, we tested whether we could identify genetic variation underlying "Crab" and "Wave" ecotypes of L. saxatilis.
resultsWe could reliably detect copy number variations, deletions and high sequence divergence (i.e. above 3%), but not single nucleotide polymorphisms. The overall hybridization pattern and number of significantly diverged genes were in close agreement with earlier phylogenetic reconstructions based on single genes. The trichotomy of L. arcana, L. compressa and L. saxatilis could not be resolved and we argue that these divergence events have occurred recently and very close in time. We found evidence for high levels of segmental duplication in the Littorina genome (10% of the transcripts represented on the array and up to 23% of the analyzed genomic fragments); duplicated genes and regions were mostly the same in all analyzed species. Finally, this method discriminated geographically distant populations of L. saxatilis, but we did not detect any significant genome divergence associated with ecotypes of L. saxatilis.
conclusionsThe present study provides new information on the sensitivity and the potential use of oligonucleotide arrays for genotyping of non-model organisms. Applying this method to Littorina species yields insights into genome evolution following the recent species radiation and supports earlier single-gene based phylogenies. Genetic differentiation of L. saxatilis ecotypes was not detected in this study, despite pronounced innate phenotypic differences. The reason may be that these differences are due to single-nucleotide polymorphisms.
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