ArticleInfection and immunity2014
Contributions of NanI sialidase to Caco-2 cell adherence by Clostridium perfringens type A and C strains causing human intestinal disease.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 45 citations in OpenAlex.
- Identification and Genomic Localization of theMicroorganisms · 2026Article
- Virulence, antimicrobial resistance, and mobile genetic elements profile ofOne health (Amsterdam, Netherlands) · 2026Article
- Sialidases as Potential Therapeutic Targets for Treatment of a Number of Human Diseases.International journal of molecular sciences · 2025Review
- Heat resistance differences are common between both vegetative cells and spores ofApplied and environmental microbiology · 2024Article
- The biology and pathogenicity ofMicrobiology and molecular biology reviews : MMBR · 2024Review
- NanJ Is the Major Sialidase for Clostridium perfringens Type F Food Poisoning Strain 01E809.Infection and immunity · 2023Article
- Characterization of NanR Regulation of Sialidase Production, Sporulation and Enterotoxin Production byToxins · 2022Article
- Sialidase Inhibitors with Different Mechanisms.Journal of medicinal chemistry · 2022Review
- NanI Sialidase Enhances the Action of Clostridium perfringens Enterotoxin in the Presence of Mucus.mSphere · 2021Article
- Pathogenicity and virulence ofVirulence · 2021Article
- NanI Sialidase Contributes to the Growth and Adherence of Clostridium perfringens Type F Strain F4969 in the Presence of Adherent Mucus.Infection and immunity · 2021Article
- Article
- Comparative in silico genome analysis of Clostridium perfringens unravels stable phylogroups with different genome characteristics and pathogenic potential.Scientific reports · 2021Article
- Phylogenomic analysis of gastroenteritis-associatedMicrobial genomics · 2019Article
- Sialidases FromFrontiers in cellular and infection microbiology · 2019Review
- NanI Sialidase Is an Important Contributor to Clostridium perfringens Type F Strain F4969 Intestinal Colonization in Mice.Infection and immunity · 2018Article
- Review
- NanR Regulates Sporulation and Enterotoxin Production by Clostridium perfringens Type F Strain F4969.Infection and immunity · 2018Article
- Enterotoxic Clostridia:Microbiology spectrum · 2018Article
- An update on the human and animal enteric pathogen Clostridium perfringens.Emerging microbes & infections · 2018Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Previous studies showed that Clostridium perfringens type D animal disease strain CN3718 uses NanI sialidase for adhering to enterocyte-like Caco-2 cells. The current study analyzed whether NanI is similarly important when type A and C human intestinal disease strains attach to Caco-2 cells. A PCR survey determined that the nanI gene was absent from typical type A food poisoning (FP) strains carrying a chromosomal enterotoxin (CPE) gene or the genetically related type C Darmbrand (Db) strains. However, the nanI gene was present in type A strains from healthy humans, type A strains causing CPE-associated antibiotic-associated diarrhea (AAD) or sporadic diarrhea (SD), and type C Pig-Bel strains. Consistent with NanI sialidase being the major C. perfringens sialidase when produced, FP and Db strains had little supernatant sialidase activity compared to other type A or C human intestinal strains. All type A and C human intestinal strains bound to Caco-2 cells, but NanI-producing strains had higher attachment levels. When produced, NanI can contribute to host cell attachment of human intestinal disease strains, since a nanI null mutant constructed in type A SD strain F4969 had lower Caco-2 cell adhesion than wild-type F4969 or a complemented strain. Further supporting a role for NanI in host cell attachment, sialidase inhibitors reduced F4969 adhesion to Caco-2 cells. Collectively, these results suggest that NanI may contribute to the intestinal attachment and colonization needed for the chronic diarrhea of CPE-associated AAD and SD, but this sialidase appears to be dispensable for the acute pathogenesis of type A FP or type C enteritis necroticans.
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