ArticleThe Journal of biological chemistry2014
Evolutionarily conserved roles of the dicer helicase domain in regulating RNA interference processing.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- The chromosome-level genome assembly of the slug Deroceras laeve facilitates its use as a comparative model of regeneration.G3 (Bethesda, Md.) · 2025Article
- Lineage-specific expansions of the Dicer gene family in tardigrades.BMC genomics · 2025Article
- The human Dicer helicase domain is capable of ATP hydrolysis and single-stranded nucleic acid binding.BMC biology · 2024Article
- Dicer-2 mutations inThe Journal of general virology · 2024Article
- Dicer structure and function: conserved and evolving features.EMBO reports · 2023Review
- Ancestral protein reconstruction reveals evolutionary events governing variation in Dicer helicase function.eLife · 2023Article
- Structural Insights into the Advances and Mechanistic Understanding of Human Dicer.Biochemistry · 2023Review
- A Tale of Two Lobsters-Transcriptomic Analysis Reveals a Potential Gap in the RNA Interference Pathway in the Tropical Rock LobsterInternational journal of molecular sciences · 2022Article
- Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases.International journal of molecular sciences · 2021Review
- Novel alternative ribonucleotide excision repair pathways in human cells by DDX3X and specialized DNA polymerases.Nucleic acids research · 2020Article
- Overexpression and purification of Dicer and accessory proteins for biochemical and structural studies.Methods (San Diego, Calif.) · 2017Article
- Molecular mechanisms of Dicer: endonuclease and enzymatic activity.The Biochemical journal · 2017Review
- Evolution of RNA- and DNA-guided antivirus defense systems in prokaryotes and eukaryotes: common ancestry vs convergence.Biology direct · 2017Review
- Structural and functional insights into the fly microRNA biogenesis factor Loquacious.RNA (New York, N.Y.) · 2016Article
- Origin of eukaryotes from within archaea, archaeal eukaryome and bursts of gene gain: eukaryogenesis just made easier?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2015Review
- Unzippers, resolvers and sensors: a structural and functional biochemistry tale of RNA helicases.International journal of molecular sciences · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The enzyme Dicer generates 21-25 nucleotide RNAs that target specific mRNAs for silencing during RNA interference and related pathways. Although their active sites and RNA binding regions are functionally conserved, the helicase domains have distinct activities in the context of different Dicer enzymes. To examine the evolutionary origins of Dicer helicase functions, we investigated two related Dicer enzymes from the thermophilic fungus Sporotrichum thermophile. RNA cleavage assays showed that S. thermophile Dicer-1 (StDicer-1) can process hairpin precursor microRNAs, whereas StDicer-2 can only cleave linear double-stranded RNAs. Furthermore, only StDicer-2 possesses robust ATP hydrolytic activity in the presence of double-stranded RNA. Deletion of the StDicer-2 helicase domain increases both StDicer-2 cleavage activity and affinity for hairpin RNA. Notably, both StDicer-1 and StDicer-2 could complement the distantly related yeast Schizosaccharomyces pombe lacking its endogenous Dicer gene but only in their full-length forms, underscoring the importance of the helicase domain. These results suggest an in vivo regulatory function for the helicase domain that may be conserved from fungi to humans.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.