Evidence map›Paper›PMID 25135320›Full record

ArticleAnalytical chemistry2014

Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products.

Xiude Hua, Wei Yin, Haiyan Shi, Ming Li, Yanru Wang, Hong Wang, Yonghao Ye, Hee Joo Kim, Shirley J Gee, Minghua Wang and 2 more

Abstract readEvaluation Study
In one paragraph

Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Xiude HuaCollege of Plant Protection (State & Local Joint Engineering Research Center of Green Pesticide Invention and Application), Nanjing Agricultural University , Nanjing 210095, P.R. China.
Wei Yin
Haiyan Shi
Ming Li
Yanru Wang
Hong Wang
Yonghao Ye
Hee Joo Kim
Shirley J Gee
Minghua Wang
Fengquan Liu
Bruce D Hammock

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
The Western Center for Agricultural Health and Safety at UC DavisU50OH007550 · OH · UNIVERSITY OF CALIFORNIA DAVIS · PI SCHENKER, MARC B · 2001 to 2010
$5.4M
NIEHS NIH HHS P42 ES004699NIEHS NIH HHS P42 ES04699NIOSH CDC HHS U50 OH007550NIOSH CDC HHS U50 OH07550
6 · The paper itself

Abstract

Two immuno-loop-mediated isothermal amplification assays (iLAMP) were developed by using a phage-borne peptide that was isolated from a cyclic eight-peptide phage library. One assay was used to screen eight organophosphorus (OP) pesticides with limits of detection (LOD) between 2 and 128 ng mL(-1). The iLAMP consisted of the competitive immuno-reaction coupled to the LAMP reaction for detection. This method provides positive results in the visual color of violet, while a negative response results in a sky blue color; therefore, the iLAMP allows one to rapidly detect analytes in yes or no fashion. We validated the iLAMP by detecting parathion-methyl, parathion, and fenitrothion in Chinese cabbage, apple, and greengrocery, and the detection results were consistent with the enzyme-linked immunosorbent assay (ELISA). In conclusion, the iLAMP is a simple, rapid, sensitive, and economical method for detecting OP pesticide residues in agro-products with no instrumental requirement.

Indexed as

Amino Acid SequenceAntibodies, MonoclonalBacteriophagesBase SequenceBrassicaDNAFood AnalysisImmunoassayLimit of DetectionMalusMolecular Sequence DataNucleic Acid Amplification TechniquesOrganophosphorus CompoundsPeptidesPesticidesAntibodies, MonoclonalDNAOrganophosphorus CompoundsPeptidesPesticides

Identifiers

PMID25135320
PMCPMC4139188

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.