ArticleThe Journal of experimental medicine2014
Classical Flt3L-dependent dendritic cells control immunity to protein vaccine.
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Article in The Journal of experimental medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64citing papers in PubMed
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Who cites it
64 citing papers in PubMed, 93 citations in OpenAlex.
- Flt3 ligand augments immune responses to anti-DEC-205-NY-ESO-1 vaccine through expansion of dendritic cell subsets.Nature cancer · 2020Trial
- Systemic clinical tumor regressions and potentiation of PD1 blockade with in situ vaccination.Nature medicine · 2019Trial
- The Proteome of Acute Muscle Pain: Observations from Acute Hypertonic-Saline-Induced Pain in Humans.International journal of molecular sciences · 2025Article
- PD-1 is requisite for skin TNature immunology · 2025Article
- FLT3LG modulates the infiltration of immune cells and enhances the efficacy of anti-PD-1 therapy in lung adenocarcinoma.BMC cancer · 2025Article
- Electroporation-mediated novel albumin-fused Flt3L DNA delivery promotes cDC1-associated anticancer immunity.Gene therapy · 2025Article
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- Pre-pregnancy obesity is associated with an altered maternal metabolome and reduced Flt3L expression in preterm birth.Scientific reports · 2024Article
- The immunoreactive signature of monocyte-derived dendritic cells from patients with Down syndrome.Clinical and experimental immunology · 2024Article
- Vaccinia Virus: Mechanisms Supporting Immune Evasion and Successful Long-Term Protective Immunity.Viruses · 2024Review
- Adjuvant DNA vaccine pNMM promotes enhanced specific immunity and anti-tumor effects.Human vaccines & immunotherapeutics · 2023Article
- Diagnostic accuracy of using multiple cytokines to predict aldosterone-producing adenoma.Scientific reports · 2023Article
- Acute effects of FLT3L treatment on T cells in intact mice.Scientific reports · 2022Article
- Fluorescent tracking identifies key migratory dendritic cells in the lymph node after radiotherapy.Life science alliance · 2022Article
- The intracellular cation channel TMEM176B as a dual immunoregulator.Frontiers in cell and developmental biology · 2022Review
- GM-CSF, Flt3-L and IL-4 affect viability and function of conventional dendritic cell types 1 and 2.Frontiers in immunology · 2022Article
- Low Level FLT3LG is a Novel Poor Prognostic Biomarker for Cervical Cancer with Immune Infiltration.Journal of inflammation research · 2022Article
- Single-cell multiomics defines tolerogenic extrathymic Aire-expressing populations with unique homology to thymic epithelium.Science immunology · 2021Article
- DNGR-1 limits Flt3L-mediated antitumor immunity by restraining tumor-infiltrating type I conventional dendritic cells.Journal for immunotherapy of cancer · 2021Article
- Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
18 authors at 3 institutions in 2 countries.
Niroshana AnandasabapathyLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Department of Dermatology/Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, MA 02115 nanandasabapathy@partners.org.
Rachel FederLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Shamim MollahLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Sze-Wah TseDepartment of Dermatology/Harvard Skin Disease Research Center, Brigham and Women's Hospital, Boston, MA 02115.
Maria Paula LonghiLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Saurabh MehandruLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Ines MatosLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Cheolho CheongLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Darren RuaneLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Lucas BraneLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Angela TeixeiraLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Joseph DobrinLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Olga MizeninaLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Chae Gyu ParkLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Matthew MeredithLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Björn E ClausenInstitute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, D-55131 Mainz, Germany.
Michel C NussenzweigLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Ralph M SteinmanLaboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065 Laboratory of Cellular Physiology and Immunology, Christopher H. Browne Center for Immunology and Immune Diseases, Hospital Informatics, and Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065.
Rockefeller University · USBrigham and Women's Hospital · USJohannes Gutenberg University Mainz · DE
Funding
Protein Expression CoreP01AI081677 · NIAID · ROCKEFELLER UNIVERSITY · PI NUSSENZWEIG, MICHEL C · 2009 to 2013
$10.6MProperties of Mouse of Dendritic Cells and MacrophagesR01AI013013 · NIAID · ROCKEFELLER UNIVERSITY · PI MOJSOV, SVETLANA · 1985 to 2015
$8.9MDendritic Cells and the Pathogenesis of HIV-1 InfectionR37AI040045 · NIAID · ROCKEFELLER UNIVERSITY · PI STEINMAN, RALPH MARVIN · 2001 to 2010
$6.1MTransforming Translational Science and Education to Benefit Human HealthKL2RR024142 · NCRR · ROCKEFELLER UNIVERSITY · PI COLLER, BARRY · 2006 to 2011
$4.5MTransforming Translational Science and Education to Benefit Human HealthKL2TR000151 · NCATS · ROCKEFELLER UNIVERSITY · PI COLLER, BARRY · 2012 to 2015
$2.1MThe clinical use of Flt3L - an immune adjuvant to potentiate Dendritic CellsK23AR063461 · NIAMS · ROCKEFELLER UNIVERSITY · PI ANANDASABAPATHY, NIROSHANA · 2012 to 2016
$726kIdentification and vaccine adjuvant responses of dendritic cells in humansR56AI040045 · NIAID · ROCKEFELLER UNIVERSITY · PI SCHLESINGER, SARAH JANE · 2011 to 2011
$520kDENDRITIC CELLS AND THE PATHOGENESIS OF HIV-1 INFECTIONR01AI040045 · NIAID · ROCKEFELLER UNIVERSITY · PI STEINMAN, RALPH M. · 1996 to 2000
$457kBritish Heart Foundation FS/13/49/30421NCATS NIH HHS KL2 TR000151NCRR NIH HHS KL2 RR024142NCRR NIH HHS RR024142NIAID NIH HHS AI40045NIAID NIH HHS N01 AI040045NIAID NIH HHS P01 AI081677NIAID NIH HHS R01 AI013013NIAID NIH HHS R01 AI040045NIAMS NIH HHS AR063461-01A1NIAMS NIH HHS K23 AR063461PHS HHS 13013PHS HHS 81677
Abstract
DCs are critical for initiating immunity. The current paradigm in vaccine biology is that DCs migrating from peripheral tissue and classical lymphoid-resident DCs (cDCs) cooperate in the draining LNs to initiate priming and proliferation of T cells. Here, we observe subcutaneous immunity is Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L is rapidly secreted after immunization; Flt3 deletion reduces T cell responses by 50%. Flt3L enhances global T cell and humoral immunity as well as both the numbers and antigen capture capacity of migratory DCs (migDCs) and LN-resident cDCs. Surprisingly, however, we find immunity is controlled by cDCs and actively tempered in vivo by migDCs. Deletion of Langerin(+) DC or blockade of DC migration improves immunity. Consistent with an immune-regulatory role, transcriptomic analyses reveals different skin migDC subsets in both mouse and human cluster together, and share immune-suppressing gene expression and regulatory pathways. These data reveal that protective immunity to protein vaccines is controlled by Flt3L-dependent, LN-resident cDCs.
Indexed as
AnimalsAntigen PresentationAntigens, SurfaceDendritic CellsFemaleGene ExpressionHumansImmunity, HumoralInjections, IntradermalInjections, SubcutaneousInterferon-gammaLectins, C-TypeLigandsMaleMannose-Binding LectinsMembrane ProteinsAntigens, SurfaceCd207 protein, mouseflt3 ligand proteinInterferon-gammaLectins, C-TypeLigandsMannose-Binding LectinsMembrane ProteinsOvalbuminProteinsTranscription FactorsVaccinesZbtb46 protein, mouse
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