ArticleScientific reports2014
Detecting protein-protein interactions based on kinase-mediated growth induction of mammalian cells.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular Association Assay Systems for Imaging Protein-Protein Interactions in Mammalian Cells.Biosensors · 2025Article
- FGFR3 drives Aβ-induced tau uptake.Experimental & molecular medicine · 2024Article
- A growth-based platform for detecting domain-peptide interactions in the cytoplasm of mammalian cells.Scientific reports · 2022Article
- Does Generic Cyclic Kinase Insert Domain of Receptor Tyrosine Kinase KIT Clone Its Native Homologue?International journal of molecular sciences · 2022Article
- Manipulating autophagic degradation in human diseases: from mechanisms to interventions.Life medicine · 2022Review
- AktAR and Akt-STOPS: Genetically Encodable Molecular Tools to Visualize and Perturb Akt Kinase Activity at Different Subcellular Locations in Living Cells.Current protocols · 2022Article
- Membrane tethering of CreER decreases uninduced cell labeling and cytotoxicity while maintaining recombination efficiency.Molecular therapy. Nucleic acids · 2022Article
- Folding and Intrinsic Disorder of the Receptor Tyrosine Kinase KIT Insert Domain Seen by Conventional Molecular Dynamics Simulations.International journal of molecular sciences · 2021Article
- Evolution of KIPPIS as a versatile platform for evaluating intracellularly functional peptide aptamers.Scientific reports · 2021Article
- Monomerization of ALK Fusion Proteins as a Therapeutic Strategy inFrontiers in oncology · 2020Article
- Bi-directional signaling by membrane-bound KitL induces proliferation and coordinates thymic endothelial cell and thymocyte expansion.Nature communications · 2018Article
- FRETting about the affinity of bimolecular protein-protein interactions.Protein science : a publication of the Protein Society · 2018Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Detection of protein-protein interactions (PPIs) is important for understanding numerous processes in mammalian cells; however, existing PPI detection methods often give significant background signals. Here, we propose a novel PPI-detection method based on kinase-mediated growth induction of mammalian cells. In this method, target proteins are fused to the intracellular domain of c-kit (c-kit ICD) and expressed in interleukin-3-dependent mammalian cells. The PPI induces dimerization and activation of c-kit ICDs, which leads to cell growth in the absence of interleukin-3. Using this system, we successfully detected the ligand-dependent homo-interaction of FKBPF36V and hetero-interaction of FKBP and FRBT2098L, as well as the constitutive interaction between MDM2 and a known peptide inhibitor. Intriguingly, cells expressing high-affinity peptide chimeras are selected from the mixture of the cell populations dominantly expressing low-affinity peptide chimeras. These results indicate that this method can detect PPIs with low background levels and is suitable for peptide inhibitor screening.
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